30,807 results
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Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG4
Plasmid#193804PurposeProtein production of ChemoG4 in bacteriaDepositorInsertChemoG4
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: E143R, E147R, L271EPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRD2A-c014
Plasmid#53626Purposebacterial expression plasmidDepositorInsertBRD2A
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
KBB609
Plasmid#185110PurposePom152 N-terminus in pEX-N-His for bacterial expression and purificationDepositorInsertPOM152
Tags6HisExpressionBacterialMutationPom152 N-term-6HisAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
ExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFC0694
Plasmid#186693PurposeShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo
ExpressionBacterialPromoterPLacAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB335 (Bcl-2 from ATG to -1281)
Plasmid#15382DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -1281 with P2Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC-eGFP
Plasmid#107408PurposeThis plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrCAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
41 pCOLI_K_NoTag
Plasmid#160265PurposeUntagged Kan resistant bacterial expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Stag-YCrs5
Plasmid#215163PurposeEncodes a yeast cadmium metallothionein fused with the S-tag at the N-terminusDepositorInsertStag-YCrs5
TagsS-tagExpressionBacterialPromoterT7 lacAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mSNX13-PX (558-677)
Plasmid#119094PurposeBacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)DepositorInsertmSNX13-PX (558-677)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only