31,185 results
-
Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
TagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOGG042
Plasmid#113998PurposeVector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectorsDepositorInsertTetracycline
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27G2)_FLAG
Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
Tags8xHis and FLAGExpressionBacterialPromoterT5Available SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (1-120)
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-09
Plasmid#79924PurposePalm3-12GS-I3-01-myc-Late1DepositorInsertEPN-09
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Dmel Or9a
Plasmid#59637PurposeProbe for Drosophila melanogaster Or9a expressionDepositorInsertOr9a (Or9a Fly)
ExpressionBacterialAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
TagsMBPExpressionBacterialPromotertacAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B2
Plasmid#74679PurposeBM3R1-B2 gateDepositorInsertPTac-RBSB2-BM3R1-PBM3R1-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mOrange LIC cloning vector (MBP-mOrange)
Plasmid#29748DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
TagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLlR5
Plasmid#149481PurposeBroad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mClover/nat
Plasmid#120330PurposeBacterial mClover labelingDepositorInsertmClover
TagsD103N (see comment below)ExpressionBacterialPromoter(None)Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-MBP-TEV-GG
Plasmid#215794PurposeCloning of target gene with N terminal MBP tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits