30,738 results

1. pgRNA-guideless

   Plasmid

   #129464

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Guideless gRNA backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBPHLWL-GAL80

   Plasmid

   #26257

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-His8-aCat(660-906)

   Plasmid

   #203333

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-906 only

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-c5X-35-H-6

   Plasmid

   #67008

   Purpose

   Expresses malE-35-H-6 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-35-H-6
   

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT937

   Plasmid

   #68936

   Purpose

   Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   Int12
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PC4_3dad1h

   Plasmid

   #70699

   Purpose

   Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

   Depositor

   Stephen Harrison

   Article

   Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

   Insert

   DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
   

   Tags

   his-tag on C-terminus of dad1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT-3

   Plasmid

   #22832

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-beta1,4 GT-1

   Plasmid

   #11872

   Depositor

   Michiko Fukuda

   Article

   Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

   Insert

   beta 1,4 galactosyltransferase (B4GALT1 Human)
   

   Expression

   Bacterial

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. ECH1

   Plasmid

   #38911

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ECH1 (ECH1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3.1-EPSIN 1

    Plasmid

    #22225

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Tags

    Xpress

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

    Plasmid

    #209292

    Purpose

    Expresses R2Bm-Cas9(H840A) fusion in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Tags

    His, MBP, SpCas9(H840A), StrepII, and bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDJSVT90

    Plasmid

    #109304

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGPΩTp

    Plasmid

    #130660

    Purpose

    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep

    Insert

    Ω trimethoprim cassette
    

    Expression

    Bacterial

    Mutation

    Addition of Ω sites next to trimethoprim cassette…

    Promoter

    dhfr promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. R

    Plasmid

    #167170

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R
    ATP1B1
    

    Expression

    Bacterial and Insect

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sup35 ScNM A230C His7

    Plasmid

    #15598

    Depositor

    Susan Lindquist

    Article

    Tessier et al Nature. 2007 May 31. 447(7144):556-61.

    Insert

    Sup35 NM (SUP35 Budding Yeast)
    

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    A230C. Alanine 230 mutated to cysteine.

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

    Plasmid

    #190237

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSNA-c023

    Plasmid

    #175469

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA221TrxA-AID-T7RNAP

    Plasmid

    #167976

    Purpose

    Expresses fusion protein TrxA-AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    TrxA-AID-T7RNAP
    

    Tags

    T7RNAP and TrxA

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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