30,738 results

1. pJF104B

   Plasmid

   #113324

   Purpose

   pTet-dCas9, pTet-MCP-SoxS_R93A

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9 and MCP-SoxS_R93A
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   R93A

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. L57R

   Plasmid

   #34557

   Depositor

   Kevin Truong

   Insert

   L57 (CASP7 Human, A.Sativa (LOV2))
   

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic domain of caspase-7 was truncated at am…

   Promoter

   CMV, T7

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET28CLY7

   Plasmid

   #21766

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pQE9-His-p97(QQ)

   Plasmid

   #14667

   Depositor

   Graham Warren

   Article

   Ye et al Nature. 2001 Dec 6. 414(6864):652-6.

   Insert

   p97 (Vcp Mouse)
   

   Tags

   RGS6xHis

   Expression

   Bacterial

   Mutation

   E305/578Q

   Available Since

   April 13, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pGEX Sh3bp2-SH2

   Plasmid

   #46484

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sh3bp2 (SH3BP2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 450-561

   Promoter

   Tac

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lbu_C2c2_E299A

   Plasmid

   #91855

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) E299A
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   E299A

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXA2 (murine) HIS-tag pET

   Plasmid

   #8557

   Depositor

   Corey Largman

   Insert

   HOXA2 (Hoxa2 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   contains 5 additional amino acids from 5' un…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. rsGFP1-pBAD

   Plasmid

   #54851

   Purpose

   Localization: Protein Expression Vector , Excitation: 493, Emission: 510. Also called rsEGFP1

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   6xHis and rsGFP1

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Tags

   His and mOrange

   Expression

   Bacterial

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b_PfSSB

    Plasmid

    #103000

    Purpose

    The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

    Depositor

    Martin Webb

    Article

    Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

    Insert

    PfSSB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_PIK3R2-1/1

    Plasmid

    #91415

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R2-1/1 (PIK3R2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TARBP1

    Plasmid

    #25505

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TARBP1 (TARBP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 10, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pBS IRES EGFP (DM#271)

    Plasmid

    #15080

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    IRES EGFP
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM-60-NusA-DmBelle_B

    Plasmid

    #145963

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Expression

    Bacterial

    Mutation

    del 155-157, T530A and I685V

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b-NAD-cpLuc

    Plasmid

    #135628

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc sensor
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-3-SH3BP1-CTD

    Plasmid

    #136649

    Purpose

    Expression of SH3BP1-CTD-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-CTD (SH3BP1 Human)
    

    Expression

    Bacterial

    Mutation

    Contains only C-terminal domain

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-3-SH3BP1-NTD

    Plasmid

    #136648

    Purpose

    Expression of SH3BP1-NTD-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-NTD (SH3BP1 Human)
    

    Expression

    Bacterial

    Mutation

    Contains only N-terminal domain

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZS-YP_009701829

    Plasmid

    #198377

    Purpose

    Express YP_009701829 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009701829 (CVA-1_407L )
    

    Expression

    Bacterial

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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