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31,943 results

Showing: 11381 - 11400 of 31943 results

  1. pGGD_RT_li_Tq2CFP

    Plasmid
    #136971
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Expression
    Bacterial
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136971-plasmid-map-sequence-id-269106

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  3. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

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  5. pY3

    Plasmid
    #218116
    Purpose
    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.
    Depositor
    Carl Denard
    Article
    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218116-plasmid-map-sequence-id-431074

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  13. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  15. p405CYC1

    Plasmid
    #15967
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15967-plasmid-map-sequence-id-8452

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  16. peSCBE3-NG-HF1

    Plasmid
    #218164
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218164-plasmid-map-sequence-id-433171

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Showing: 11381 - 11400 of 31943 results