30,738 results

1. RGS1

   Plasmid

   #39143

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RGS1 (RGS1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCHAP4

   Plasmid

   #206850

   Purpose

   A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.

   Depositor

   Bogumil Karas

   Article

   Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBR1_DVK_AE(J23100-B0034m-E0040m-B0015)

   Plasmid

   #197659

   Purpose

   Expresses GFP under the control of J23100 and B0034m

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA790

   Plasmid

   #186245

   Purpose

   dnap-C HR Donor Vector (T4 phage editing)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   BBR1 backbone + T4 phage homology
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-BRD (1079-1198)

   Plasmid

   #99507

   Purpose

   Bacterial expression of mouse CBP bromodomain

   Depositor

   Peter Wright

   Article

   Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

   Insert

   mouse CBP BRD (1079-1198) (Crebbp Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry-LOV2 C450A

   Plasmid

   #81031

   Purpose

   Mammalian expression of the dark state of LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV2
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   C450A

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCiK5

   Plasmid

   #167514

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-mKelly2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-1-3xAU1

   Plasmid

   #129572

   Purpose

   Empty backbone for AU1 tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Tags

   3xAU1 and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM41-EcPPXc

   Plasmid

   #38329

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available Since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. GST TopBP1 D (aa 1-1013)

    Plasmid

    #20374

    Depositor

    Aziz Sancar

    Article

    Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

    Insert

    TopBP1 (1-1013) (TOPBP1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 1-1013

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. SMARCA2

    Plasmid

    #73250

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SMARCA2B (SMARCA2 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Terminase_T7

    Plasmid

    #188835

    Purpose

    Large terminase from phage T7

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Terminase_HHV8

    Plasmid

    #188858

    Purpose

    Large terminase from human herpesvirus 8

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. tISR

    Plasmid

    #122664

    Purpose

    Express tISR protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tISR (OCLM Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pQFT-lacZ

    Plasmid

    #208896

    Purpose

    pQFT with lacZ under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Insert

    lacZ
    

    Expression

    Bacterial

    Mutation

    Contains two silent mutations and a missense muta…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM-and1 in situ probe

    Plasmid

    #52102

    Purpose

    actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

    Depositor

    Didier Stainier

    Article

    Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

    Insert

    actinodin 1 in situ probe (and1 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-nTR

    Plasmid

    #21812

    Depositor

    Wafik El-Deiry

    Article

    Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

    Insert

    pQE-nTR (TNFSF10 Human)
    

    Expression

    Bacterial

    Mutation

    His tag and aa95-113 were removed from pQE-hTR

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pET-DEST42-ARF1-V5-His6

    Plasmid

    #67411

    Purpose

    Expression of hArf1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. TIF1A

    Plasmid

    #74647

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TIF1A (TRIM24 Human)
    

    Tags

    His-6-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only