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B6-1

Plasmid

#111485

Purpose

Phe-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Phe-tRNA synthetase B

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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HS89 pComb3xss anti-Homer1 nanobody

Plasmid

#134717

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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L-RARS-5

Plasmid

#166535

Purpose

scFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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L-KARS-5

Plasmid

#166530

Purpose

scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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O-DARS-1

Plasmid

#166538

Purpose

scFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-length

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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O-LARS-15

Plasmid

#166542

Purpose

scFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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p264 pSP72 hAR-LBD(LNCaP)

Plasmid

#12495

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Expression

Bacterial

Mutation

Thr 878 Ala in AR-LBD

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pJUMP19-Pac

Plasmid

#126957

Purpose

Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJ128

Plasmid

#19962

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Article

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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GFP Q157TAG

Plasmid

#68297

Purpose

Q157TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP Q157TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL7026

Plasmid

#128811

Purpose

Fluoride riboswitch-regulated 3-way junction dimeric broccoli

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated 3-way junction dimeric broccoli

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBMPGAL4LWL

Plasmid

#26270

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pBluescript goosecoid

Plasmid

#16935

Depositor

John Postlethwait

Article

Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

Insert

goosecoid (gsc Zebrafish)

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
B6-2

Plasmid

#111486

Purpose

Pro-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Pro-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E/S129E

Plasmid

#36064

Depositor

Hilal Lashuel

Insert

alpha-synuclein S87E/S129E (SNCA Human)

Expression

Bacterial

Mutation

S87E/S129E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pNP555

Plasmid

#222722

Purpose

sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC130

Plasmid

#216364

Purpose

xhNup98-Nb3i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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