Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,457 results

Showing: 11521 - 11540 of 32457 results

  1. pHIT179

    Plasmid
    #204510
    Purpose
    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a (C. elegans) (csr-1 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204510-plasmid-map-sequence-id-403822

    Enlarge

  8. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

    Enlarge

  9. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

    Enlarge

  15. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113397-plasmid-map-sequence-id-217374

    Enlarge

  16. pELlR1

    Plasmid
    #172569
    Purpose
    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLlacO-1
    Available Since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    172569-plasmid-map-sequence-id-347325

    Enlarge

  17. pELlR2

    Plasmid
    #172555
    Purpose
    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLlacO-1
    Available Since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    172555-plasmid-map-sequence-id-346737

    Enlarge

  18. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid
    #101211
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101211-plasmid-map-sequence-id-198053

    Enlarge

  19. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

    Enlarge

  20. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

    Enlarge

Showing: 11521 - 11540 of 32457 results