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pQE81XN-mGold

Plasmid

#218959

Purpose

Expresses Hisx6-Tagged mGold in bacteria

Depositor

Benjamin Glick

Insert

mGold

Tags

6X-His

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx M-Ras(G22C) CL

Plasmid

#224268

Purpose

Protein purification of M-Ras (G22C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Expression

Bacterial

Mutation

G22C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx Rit1(G47C) CL

Plasmid

#224267

Purpose

Protein purification of Rit1 (G47C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rit1 (RIT1 Human)

Expression

Bacterial

Mutation

G47C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx Ypt1(S17C) CL

Plasmid

#224265

Purpose

Protein purification of Ypt1 (S17C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Ypt1 (YPT1 Budding Yeast)

Expression

Bacterial

Mutation

S17C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx Rap1A(G12C, L96F) CL

Plasmid

#224264

Purpose

Protein purification of Rap1A (G12C, L96F)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Expression

Bacterial

Mutation

G12C, L96F

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPO3

Plasmid

#209977

Purpose

Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (pAMBeta1)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-ptetO::bac::gfp

Plasmid

#217882

Purpose

expresses the bac-BGC in bacterial cells, produces bacillamide D

Depositor

Tobias A. M. Gulder

Article

Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

Inserts

Dehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase

Expression

Bacterial

Promoter

ptetO

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1m-ELP

Plasmid

#170763

Purpose

EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

EF1m-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDM030

Plasmid

#216810

Purpose

Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Type

Empty backbone

Use

CRISPR; Fungal expression

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM052

Plasmid

#216814

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Article

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264_wt-His6

Plasmid

#220214

Purpose

Bacterial expression of wild type TEX264, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-Ubiquitin

Plasmid

#223236

Purpose

Bacterial expression construct for GST-Tagged Ubiquitin production and purification

Depositor

Weixing Zhao

Insert

Ubiquitin

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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