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LexA-GFP

Plasmid

#220157

Purpose

To track the natural LexA promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

LexA promoter only

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Fur-GFP

Plasmid

#220156

Purpose

To track the natural Fur promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Fur promoter only

Expression

Bacterial

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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PhoB-GFP

Plasmid

#220163

Purpose

To track the natural PhoB promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

PhoB promoter only

Expression

Bacterial

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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FlhD-GFP

Plasmid

#220154

Purpose

To track the natural FlhD promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

FlhD promoter only

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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Fnr-GFP

Plasmid

#220155

Purpose

To track the natural Fnr promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Fnr promoter only

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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PhoP-GFP

Plasmid

#220164

Purpose

To track the natural PhoP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

PhoP promoter only

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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NarL-GFP

Plasmid

#220160

Purpose

To track the natural NarL promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NarL promoter only

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJB4

Plasmid

#177656

Purpose

Recombinant protein synthesis of human DNAJB4

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB4 (DNAJB4 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRhaQCas-BsaI

Plasmid

#170631

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Expression

Bacterial

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSPYngK+-hp

Plasmid

#48121

Purpose

Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

codon optimized signal peptide of YngK

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb92

Plasmid

#159424

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb92

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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HARS_pNIC-Bio3

Plasmid

#153050

Purpose

Expression construct for producing Avi tagged histidyl tRNA synthetase

Depositor

Susanne Gräslund

Insert

HARS (HARS1 Human)

Tags

Avi and His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pD2_miniluc_newP7

Plasmid

#174105

Purpose

adding the mini luciferase and mini promoter to the MPRA lenti construct

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Insert

mini luciferase mini-promoter

Expression

Bacterial

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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plYJP067-Ptet

Plasmid

#202299

Purpose

PTet-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Ptet-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCosSAD1AprR

Plasmid

#190786

Purpose

An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

upstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS SOHo1 (CC#99)

Plasmid

#15132

Depositor

Connie Cepko

Article

Schulte et al Development. 2000 Dec . 127(23):5033-45.

Insert

SOHo1 (SOHO-1 Chicken)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only