29,981 results

1. pBBR6k-J23109-GFPuv

   Plasmid

   #106392

   Purpose

   pBBR6k-GFPuv with Anderson promoter J23109 expressing lacI

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6272 pDEST-60-GST-HPV16E6

   Plasmid

   #24127

   Depositor

   Peter Howley

   Insert

   HPV16 E6 (E6, E6 HPV)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   none

   Promoter

   Available since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pET28b-PU.1-ETS

   Plasmid

   #85734

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
   

   Use

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT221

   Plasmid

   #134889

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LDB204

   Plasmid

   #185426

   Purpose

   Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

   Depositor

   Ken Belanger, Laura Davis

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 FXFG-repeats domain expressed

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. RP1B FUS 1-163 QQ4xSS #2

   Plasmid

   #127195

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 QQ4xSS #2 (FUS Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTU2S-b (colE1 origin)

   Plasmid

   #74092

   Purpose

   Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Mutation

   Promoter

   Potef

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-KpDcr1

   Plasmid

   #32022

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pASKt15C+SrtA

    Plasmid

    #64983

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRset-ARSeNL

    Plasmid

    #207872

    Purpose

    Bacterial expression of the BRET ATP sensor

    Depositor

    Mathew Tantama

    Article

    Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

    Insert

    ARSeNL
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBLlSh2

    Plasmid

    #170981

    Purpose

    Expression of ShCAST under control of lacI Plac promoter/repressor pair.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Inserts

    lacI
    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlac01

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAB3893

    Plasmid

    #47844

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 13 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSM12

    Plasmid

    #101643

    Purpose

    Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    meffCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge