33,906 results
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Plasmid#234683PurposeGolden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiTDepositorTypeEmpty backboneTagsHis6-3C-3XFLAG-SUMO and Thrombin-HiBiTExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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p354_DG
Plasmid#234697PurposeGolden gate domesticated pET28b for scarless cloning with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p359_DG
Plasmid#234702PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3CDepositorTypeEmpty backboneTagsHis10-mNeongreen-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p347_DG
Plasmid#234677PurposeGolden gate domesticated pET28b with His10-ThrombinDepositorTypeEmpty backboneTagsHis10-ThrombinExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL31
Plasmid#227428PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRExpressionBacterialAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#227429PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRExpressionBacterialAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRecE564T
Plasmid#226825PurposeExpressing RecE*T controled by Ptrc promoterDepositorInsertrecE*T
ExpressionBacterialAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISFba1
Plasmid#226820PurposeExpressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 oriDepositorInsertISFba1
ExpressionBacterialAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG050
Plasmid#231319PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmRDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80RFP-pXD68Kan2-RFP
Plasmid#225329PurposeRSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistanceDepositorInsertRFP
ExpressionBacterialAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core
Plasmid#233618PurposeExpression of His-tagged helicase core region of DDX3X in E.coliDepositorAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialPromoterpBADAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Octo_VirD2_NLS-Cas9-6xHis
Plasmid#232296PurposeExpresses Cas9 protein fused with octopine-type VirD2-originating NLSDepositorInsertOcto_VirD2_NLS-Cas9-6xHis
UseCRISPRTags6xHis and Octopine-type VirD2-derived NLSExpressionBacterialPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1-psHaloTag1a
Plasmid#232940PurposePhotoswichable HaloTag: psHaloTag1a variantDepositorInsertphotoswitchable HaloTag 1a variant
TagsHis6x-HRV3CExpressionBacterialMutationE143W, A258W with linkers L1 (FAG) and L2 (P)PromoterT7Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only