29,981 results

1. pAW204

   Plasmid

   #113244

   Purpose

   E. coli expression vector for IHFa F13A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa F13A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F13A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATTYA

   Plasmid

   #42412

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ATTYA (TAT Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pBbE5k-RFP

   Plasmid

   #35279

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS101

   Plasmid

   #118280

   Purpose

   Expresses GFPmut2 with low cell-to-cell variation on a low-copy plasmid and tetracycline induction

   Depositor

   Zach Hensel

   Article

   Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX/PD-L1 3SA

   Plasmid

   #121467

   Purpose

   Expression of GST-human PD-L1 in E. Coli

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S176A, T180A, S184A

   Promoter

   Tac

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSM17

   Plasmid

   #101641

   Purpose

   Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   eqFP486
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMB1-D1-TelR

   Plasmid

   #204983

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPO635

   Plasmid

   #195297

   Purpose

   pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABC operon of McCAST (Tn7575)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG inducible lac promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-24a-CXCR4

   Plasmid

   #133431

   Purpose

   Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b-Strep-hAGT-His

    Plasmid

    #167276

    Purpose

    Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    hAGT (AGT Human)
    

    Use

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. O-IL8-15

    Plasmid

    #166544

    Purpose

    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSVD5

    Plasmid

    #18877

    Depositor

    Jay Keasling

    Article

    Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

    Insert

    exopolyphosphatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pE-SUMO CRYBA3

    Plasmid

    #80747

    Purpose

    Expression of human beta A3-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYBA3 (CRYBA1 Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC13

    Plasmid

    #50003

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEXNKI-GST-3C-LIC

    Plasmid

    #108710

    Purpose

    Expression of a GST-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    GST-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. zw3 (shaggy) pbOVI

    Plasmid

    #38749

    Depositor

    Norbert Perrimon

    Article

    Siegfried et al Nature. 1990 Jun 28;345(6278):825-9.

    Insert

    zw3 (sgg Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pmcsg7-mCRT(WT)

    Plasmid

    #83505

    Purpose

    Bacterial expression of Calreticulin

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSA10(gfcD)

    Plasmid

    #176191

    Purpose

    gfcD protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSA10(gfcD-10xHis)

    Plasmid

    #176192

    Purpose

    gfcD protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge