33,480 results
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Plasmid#221341PurposeExpress ChmI in E. coliDepositorInsertchmI
TagsHis6ExpressionBacterialAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRExpressionBacterial and MammalianPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
ExpressionBacterial and YeastAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
ExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorTags6xHis-TEVExpressionBacterialPromoterT7Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
TagsHis6 and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#221134PurposeContains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminatorDepositorInserts[PibpA-gfpASV]
[PcpxPmcherryASV] OPT
ExpressionBacterialPromotercpxP and ibpAAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac EcW-TGA-h9
Plasmid#218765Purposeencodes EcWRS-h9 and EcW-tRNA-TGADepositorInsertEcWRS-h9
ExpressionBacterialMutationS8A, V144S, V146APromotertacIAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.1t
Plasmid#210254PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCerulean
superfolder GFP
TagsssraExpressionBacterialPromoteripaH7.8p and rpsMAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEmpty
Plasmid#214051PurposeEmpty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TJF229
Plasmid#132575PurposepZE1-oxySp-mCherry-proD-oxyRDepositorInsertsoxySp-mCherry-TermT1
proD-oxyR-TermT1
ExpressionBacterialPromoteroxySp and proDAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits