31,059 results
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Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
ExpressionBacterialMutationN/APromoterN/AAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)
Plasmid#48292DepositorTypeEmpty backboneTagsHis6 and ThioredoxinExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS415-KEX2
Plasmid#10996DepositorAvailable SinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPIN4
Plasmid#73239PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST TCF4 DBD
Plasmid#16488DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUdO4t
Plasmid#210249Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO-N
Plasmid#128143PurposeDinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marinaDepositorInsertRifampin ADP-ribosylating transferase + potential promoter
ExpressionBacterialAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3992 pET ubcH5
Plasmid#8652DepositorInsertUbcH5 (UBE2D1 Human)
ExpressionBacterialAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJT106b3
Plasmid#31393DepositorInsertred light inverter with lacZ output
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
TLS 4: hTLS-CHOP.pBSKS(+)
Plasmid#21830DepositorInsertTLS-CHOP (FUS Human)
ExpressionBacterialAvailable SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyExpressionBacterialPromoterPTACAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pChlr-pNEB193
Plasmid#52136PurposeA backbone plasmid for creation of additional PUF repeat modulesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Endophilin SH3 domains
Plasmid#47401DepositorInsertEndophilin
TagsGSTExpressionBacterialMutationSH3 domain (aa 292-352)PromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src42a
Plasmid#126673PurposeExpression of GST-Src42a for purification from E. coliDepositorInsertSrc42a
ExpressionBacterialAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL4672 (pEffector_TnpB3,ωRNA)
Plasmid#210872PurposeExpresses GstTnpB3 and native ωRNA from constitutive promotersDepositorInsertGstTnpB3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETa(+) HpCsd3 (42-403)
Plasmid#71285PurposeExpresses Csd3 from Helicobacter pyloriDepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only