33,906 results
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Plasmid#227644PurposeVibrio harveyi acyl-ACP synthetaseDepositorInsertAcyl-Acyl carrier protein synthetase
Tags10xHisExpressionBacterialPromoterT7Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry
Plasmid#229146PurposePromoter strength evaluation of PtlpADepositorInsertmCherry
ExpressionBacterialMutationNoPromoterPtlpAAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7_zntR
Plasmid#216324PurposeT7 promoter driving expression of zntRDepositorInsertZinc-responsive activator
ExpressionBacterialAvailable sinceDec. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCN34e-ktrA-yfp
Plasmid#227640Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertktrA-yfp
ExpressionBacterialAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-kimA-yfp
Plasmid#227638Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14
Plasmid#213518PurposeExpresses NSP14 of SARS-CoV-2DepositorAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-T4uvsX
Plasmid#228213PurposeExpression in BL21(DE3) of T4 recombinaseDepositorInsertuvsX (uvsX Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HaloKbp1b
Plasmid#228794PurposeChemigenetic indicator for potassium ion - low affinityDepositorInsertHaloKbp1b
TagsHis-TagExpressionBacterialAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-7)-neg_tag1
Plasmid#219419PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-7)-neg_tag1
TagsDEEEDD and MGHHHHHGGExpressionBacterialPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC8
Plasmid#224208PurposeExpresses human KDAC8 (HDAC8) in E. coliDepositorAvailable sinceDec. 6, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGD289
Plasmid#228113PurposepET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoterDepositorInsertKva1 retron, ncRNA with 90 bp rpoB donor
ExpressionBacterialPromoterT7Available sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW358f
Plasmid#227935PurposeExpress hIAPP-T7nDepositorInserthIAPP-T7n
ExpressionBacterialAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW357b
Plasmid#227930PurposeLow-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632SAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW357bN
Plasmid#227932PurposeAccessory plasmid for negative selectionDepositorInsertsgIII-neg
T7c
ExpressionBacterialMutationR632SAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW357d5
Plasmid#227931PurposeHigh-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632S, Q649SAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CBE-sgRNA
Plasmid#221137PurposeHelper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with Cas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
Tags6X-HisExpressionBacterialPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM236 Ubc4
Plasmid#226361PurposeExpression of yeast Ubc4DepositorAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR_Lux
Plasmid#226457Purposelambda immunity region to the luciferase operon (lux)DepositorInsertlambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
ExpressionBacterialAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only