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pTn7-SCOUT22.RY

Plasmid

#201018

Purpose

pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.YB

Plasmid

#201019

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT10

Plasmid

#200989

Purpose

pL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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M13cp-dg1 hp

Plasmid

#218093

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI

Depositor

Kevin Esvelt

Publication

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJK503

Plasmid

#155380

Purpose

dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Publication

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available since

May 4, 2024

Availability

Academic Institutions and Nonprofits only

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pR6K-GFP-CASTIF

Plasmid

#199653

Purpose

R6K plasmid encoding a type I-F CAST but no CRISPR array

Depositor

IIya Finkelstein

Insert

CAST I-F systems

Expression

Bacterial

Promoter

J23119 promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pR6K-GFP-RFP

Plasmid

#199652

Purpose

R6K backbone with Golden Gate (GG) cloning sites

Depositor

IIya Finkelstein

Type

Empty backbone

Expression

Bacterial

Promoter

J23119 promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV104

Plasmid

#215310

Purpose

Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Publication

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PAraBAD

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1

Plasmid

#218817

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Publication

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Bacterial

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1.1

Plasmid

#218818

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Publication

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Bacterial

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1.2

Plasmid

#218819

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Publication

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Expression

Bacterial

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-DiLACCO1

Plasmid

#218820

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate control biosensor

Depositor

Robert Campbell

Publication

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

DiLACCO1

Expression

Bacterial

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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ReU_pET29b

Plasmid

#215426

Purpose

Expression construct for ReU gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ReU

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NylCA_pET28a

Plasmid

#215418

Purpose

Expression construct for NylCA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NylCp2_pET28a

Plasmid

#215420

Purpose

Expression construct for NylCp2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Publication

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY2

Plasmid

#218115

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

Depositor

Carl Denard

Publication

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-THRX-TEV-GG

Plasmid

#215796

Purpose

Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-SUMO1-GG

Plasmid

#215799

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-GST-TEV-GG

Plasmid

#215801

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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