30,733 results

1. pQTEV-FBXO31

   Plasmid

   #34815

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   F-box protein 31 (FBXO31 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOLUC

   Plasmid

   #12514

   Depositor

   Tong-Chuan He

   Article

   Feng et al Biotechniques. 2002 May . 32(5):992, 994

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_THRB_Hormone-recep

   Plasmid

   #109819

   Purpose

   Protein expression and purification of THRB_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   THRB_Hormone-recep (THRB Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-CA TAG20

   Plasmid

   #105666

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 20

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBA1018

   Plasmid

   #189587

   Purpose

   T4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   T4wtGT7 Deletion Locus 250bp Homology Arms
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Encodes for a full deletion of T4 from 52.1 (part…

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB1-B-TelR

   Plasmid

   #204980

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAPDHS

   Plasmid

   #39010

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GAPDHS (GAPDHS Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. ARHGAP11AA

   Plasmid

   #25261

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ARHGAP11AA:M1-L253 (ARHGAP11A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids M1-L253

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. CYP11A1 (3N9Y)

    Plasmid

    #28119

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

11. GST-PS-Sos1 (PRM) - Cys (XSB364)

    Plasmid

    #78506

    Purpose

    Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Sos1 -Cys (PRM) (SOS1 Human)
    

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA200

    Plasmid

    #74248

    Purpose

    pGPD1-dPSTRy(4,3)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pGPD1-dPSTRy(4,3)
    

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-pr-mEos4b (mEosFP4b-V69T)

    Plasmid

    #99226

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4b
    

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 141_pT3TS_iCre

    Plasmid

    #171793

    Purpose

    Plasmid for optimised iCre RNA synthesis using T3 promoter.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised Cre for RNA expression
    

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSumo-His6- Sumo-AtTPL(1-209)

    Plasmid

    #177856

    Purpose

    Bacterial Expression of TPL

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPL (TPL Mustard Weed)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-parkin 77-465

    Plasmid

    #45970

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 77-465 (Prkn Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTL-1

    Plasmid

    #22805

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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