30,733 results

1. pETM41-EcLonA

   Plasmid

   #38330

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   Lon protease fragment (lon Escherichia coli)
   

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Polyphosphate binding fragment of Lon protease. …

   Promoter

   T7

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBAD-iGECInano

   Plasmid

   #186189

   Purpose

   Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

   Insert

   iGECInano
   

   Expression

   Bacterial

   Promoter

   AraC

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO5-SgHottip-GFP-CRISPRa

   Plasmid

   #134990

   Purpose

   dCas9-mediated activation of HOTTIP in mammalian cells

   Depositor

   Suming Huang

   Article

   Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

   Insert

   HOTTIP (HOTTIP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX6p1 AO7RE

   Plasmid

   #137843

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DJ-1 TEV site pET15b

   Plasmid

   #60687

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   TEV site was inserted (see comments below).

   Promoter

   T7

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pClodANL-Cherry

   Plasmid

   #107870

   Purpose

   Bacterial expression of cherry-NanoLuc

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   Cherry (red fluorescent protein)
   

   Use

   Luciferase

   Tags

   NanoLuc

   Expression

   Bacterial

   Promoter

   pro D

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4136463

   Plasmid

   #149527

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCA528-AMOT(1-300)-sortase

   Plasmid

   #166706

   Purpose

   expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   AMOT aa 1-300
   

   Tags

   His-SUMO and Sortase tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-pvdA WT

   Plasmid

   #87098

   Purpose

   expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

   Insert

   pvdA WT
   

   Tags

   his-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE2-TetOminiCMV-SK

    Plasmid

    #136577

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EKAR2G_design1_mTFP_159_Venus_173

    Plasmid

    #39825

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EKAR2G_design1_mTFP_175_Venus_195

    Plasmid

    #39831

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pBT3019

    Plasmid

    #122541

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::SKL
    

    Tags

    SKL

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EKAR2G_design1_mTFP_105_Venus_195

    Plasmid

    #39821

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-kanMX6-P81nmt1-mYFP

    Plasmid

    #105144

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P81nmt1-mYFP
    

    Tags

    81nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    81nmt1

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p32m3C Trx-CC

    Plasmid

    #196475

    Purpose

    Protein expression of Thiolredoxin-3C cleavage site-CarHCC

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    Trx-CC
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Pf-GMPS

    Plasmid

    #36899

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-GMPS (PF10_0123 Plasmodium falciparum)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. SAT

    Plasmid

    #25493

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SAT (SAT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. BASIC_7_J23101-RBS34-mCherry-B0015

    Plasmid

    #68141

    Purpose

    storage vector for BASIC part: RFP expression cassette

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    J23101-RBS34-mcherry-B0015
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PDLIM5

    Plasmid

    #38811

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PDLIM5 (Pdlim5 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only