29,955 results

1. pC23-LIC-STREP

   Plasmid

   #182507

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal STREP

   Depositor

   Renato Bruni

   Type

   Empty backbone

   Use

   Tags

   TEV-STREP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CUL3A-c013

   Plasmid

   #53672

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   CUL3A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   solubilizing mutations I342R & L346D

   Promoter

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pT-43

   Plasmid

   #16232

   Depositor

   Yoshiko Kikuchi

   Article

   Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

4. MUT

   Plasmid

   #38872

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MUT (Mut Human)
   

   Use

   Tags

   Tev His Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDUAL_2_T5

   Plasmid

   #155175

   Purpose

   see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RECQL5 (RECQL5A-c011)

   Plasmid

   #98231

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RECQL5A (RECQL5 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. K560CLM T324C

   Plasmid

   #24460

   Depositor

   Ron Vale

   Article

   Yildiz et al Science. 2004 Jan 30. 303(5658):676-8.

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S T324C/C421A

   Promoter

   Available since

   Aug. 12, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCph8

   Plasmid

   #50552

   Purpose

   Expresses Cph8 under a modified PLtetO-1 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Insert

   Cph8
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET21a Hbb

    Plasmid

    #26639

    Depositor

    Phoebe Rice

    Article

    Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

    Insert

    Hbb (hbb Borrelia burgdorferi)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHH0103_YWHAH_14-3-3

    Plasmid

    #109988

    Purpose

    Protein expression and purification of YWHAH_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAH_14-3-3 (YWHAH Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZMYND8 (PRKCBP1A-c097)

    Plasmid

    #98244

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PRKCBP1A (ZMYND8 Human)
    

    Use

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BPV00605 pET21GG2-His(6)-BECN(1-265)

    Plasmid

    #136686

    Purpose

    Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 IDR + CC (BECN1 Human)
    

    Use

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid

    #15405

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    insert can be released by digestion with REs NcoI…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJAT13araE

    Plasmid

    #18987

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    araE (araE E.coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G06_ZIKV NS1

    Plasmid

    #145352

    Purpose

    Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS1
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJOE6227.1

    Plasmid

    #135078

    Purpose

    An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

    Depositor

    Josef Altenbuchner

    Article

    Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

    Insert

    up- and downstream regions of upp (upp Pseudomonas putida)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge