29,955 results

1. PDLIM7_PDZ_1

   Plasmid

   #103940

   Purpose

   Protein expression and purification of ENIGMA PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDLIM7 (PDLIM7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJAT13

   Plasmid

   #18986

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   promoter CP13
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEB1-Venus SX

   Plasmid

   #103990

   Purpose

   Plasmid encoding Venus SX

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Venus SX
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F46L, F64L, …

   Promoter

   proC

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEB2-2ndVal Kat9-5

   Plasmid

   #104031

   Purpose

   Plasmid encoding 2ndVal Katushka9-5

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal Katushka9-5
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Katushka9-5 with valine inserted in 2nd position

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEB2-2ndVal mCherry-L

   Plasmid

   #104026

   Purpose

   Plasmid encoding 2ndVal mCherry-L

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mCherry-L
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   mCherry-L with valine inserted in 2nd position

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEB1-coSCFP1

   Plasmid

   #104014

   Purpose

   Plasmid encoding coSCFP1

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   coSCFP1
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Same protein sequence as SCFP1 (codon optimized n…

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEB1-comVenJBC

   Plasmid

   #104020

   Purpose

   Plasmid encoding comVenJBC

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   comVenJBC
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Same protein sequence as mVenus JBC (codon optimi…

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEB1-comVenME

    Plasmid

    #104021

    Purpose

    Plasmid encoding comVenME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    comVenME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Same protein sequence as mVenus ME (codon optimiz…

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEB1-coClover

    Plasmid

    #104022

    Purpose

    Plasmid encoding coClover

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    coClover
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Same protein sequence as Clover (codon optimized …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEB1-comClover3

    Plasmid

    #104023

    Purpose

    Plasmid encoding comClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    comClover3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Same protein sequence as mClover3 (codon optimize…

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB1-mVenus JBC

    Plasmid

    #103987

    Purpose

    Plasmid encoding mVenus JBC

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus JBC
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEB1-SCFP1

    Plasmid

    #103969

    Purpose

    Plasmid encoding SCFP1

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    SCFP1
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_PFN2_Profilin

    Plasmid

    #110007

    Purpose

    Protein expression and purification of PFN2_Profilin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PFN2_Profilin (PFN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCri-8a

    Plasmid

    #61317

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pEC-K-CBP_CjeCas9

    Plasmid

    #83043

    Purpose

    Expresses Campylobacter jejuni Cas9 (Cje Cas9).

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CjeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGR-ECK120015170

    Plasmid

    #46006

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120015170 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSG lacZ

    Plasmid

    #63874

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    +1 frameshift near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. dps-RFP

    Plasmid

    #63843

    Purpose

    This plasmid is designed as a bacterial oxidative stress sensor

    Depositor

    Howard Salis

    Insert

    dps, mRFP1 and GFPmut3b
    

    Use

    Tags

    mRFP1

    Expression

    Bacterial

    Mutation

    Gene is transcriptionally fused to RFP

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge