31,572 results
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Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only
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pCDF-KpAvs4
Plasmid#188807PurposeCodon optimized KpAvs4 with C-terminal His tagDepositorInsertKpAvs4
TagsHisExpressionBacterialPromoterT7Available SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-EcAvs2
Plasmid#188804PurposeCodon optimized EcAvs2 with C-terminal His tagDepositorInsertEcAvs2
TagsHisExpressionBacterialPromoterT7Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT14.G
Plasmid#201002PurposepLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApRDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CN-B
Plasmid#196477PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCNDepositorInsertB-CN-B
ExpressionBacterialPromoterT7Available SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
KALRN (KALRNA-c001)
Plasmid#110256PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-4xDQNAT
Plasmid#128391PurposeIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sitesDepositorInsertProtein D
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SF3B1-NTerm
Plasmid#97425PurposeExpresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coliDepositorInsertSF3B1-N-term (AA1-500)
TagsGSTExpressionBacterialMutationSilent mutation at nt876, aa289 (AGG to AGA)PromotertacAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2
Plasmid#140687Purpose3-color stripe (different sgRNAs)DepositorInsert3-color stripe (different sgRNAs)
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Terminase_T7
Plasmid#188835PurposeLarge terminase from phage T7DepositorInsertTerminase
ExpressionBacterialAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Terminase_HHV8
Plasmid#188858PurposeLarge terminase from human herpesvirus 8DepositorInsertTerminase
ExpressionBacterialAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b GFP-Rab10 Q68LHis
Plasmid#186015PurposeGFP-Rab10 Q68LHisDepositorInsertGFP-Rab10 Q68LHis (RAB10 Human)
ExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH75
Plasmid#217383PurposePtrc99A-mCherry-L6-PopTag for bacterial expressionDepositorInsertPopTag
TagsmCherry-Linker6ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH80
Plasmid#217387PurposePtrc99A-mCherry-L78-PopTag for bacterial expressionDepositorInsertPopTag
TagsmCherry-Linker78ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISAba30_reRNA
Plasmid#205323PurposeBacterial expression of ISAba30 TnpB and its reRNADepositorInsertISAba30 TnpB
ExpressionBacterialAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
ExpressionBacterialMutationSee depositor's comments below.Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only