30,695 results

1. HNRNPH2_3xRRM_pGEX

   Plasmid

   #135115

   Purpose

   Recombinant expression of HNRNPH2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   HNRNPH2 (HNRNPH2 Human)
   

   Tags

   GST-SBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIA1405

   Plasmid

   #172404

   Purpose

   Expression of SARS-COV-2 Nsp12 D218A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   D218A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIA1420

   Plasmid

   #172406

   Purpose

   Expression of SARS-COV-2 Nsp12 R116A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   R116A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIA1421

   Plasmid

   #172407

   Purpose

   Expression of SARS-COV-2 Nsp12 K50A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-MK4(6)

   Plasmid

   #30189

   Depositor

   Bruce Tempel

   Insert

   Kcna6 (Kcna6 Mouse)
   

   Expression

   Bacterial

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET21-10XHis-GST-HRV-Her2-dL5-Her2

   Plasmid

   #85624

   Purpose

   Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

   Depositor

   Marcel Bruchez

   Insert

   10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)
   

   Tags

   10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …

   Expression

   Bacterial

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   T7

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-DDX43-K292A

   Plasmid

   #134575

   Purpose

   Express human DDX43 mutant (K292A, ATPase dead) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-K292A (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (K292A)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMtb CblA

   Plasmid

   #138298

   Purpose

   Expression of Mtb CblA

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Mtb CblA
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMtb ATR

    Plasmid

    #138297

    Purpose

    Expression of Mtb ATR

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Corrinoid adenosyl transferase
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. RHEB

    Plasmid

    #55683

    Purpose

    expression of mouse RHEB

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RHEB (Rheb Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET His6 MBP TEV expression vector with BioBrick polypromoer restriction sites (14-C)

    Plasmid

    #48309

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Smad3 LC

    Plasmid

    #12633

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

    Insert

    Smad3 LC (SMAD3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LC segment of Smad3 (aa 146-425)

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET28-SUMO-KaiA

    Plasmid

    #68049

    Purpose

    Strain: AM5115, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiA
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nsp3 Mac1 - C2 (UCSF)

    Plasmid

    #169209

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pHH0103_ABL1-1/1

    Plasmid

    #91521

    Purpose

    Protein expression and purification of human SH3 domain construct ABL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ABL1-1/1 (ABL1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CDKL1_HUMAN_D0

    Plasmid

    #79699

    Purpose

    This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDKL1 (CDKL1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. GPX1

    Plasmid

    #38864

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GPX1 (Gpx1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    SeCys49 -> Gly

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only