30,307 results

1. pE-SUMO CRYBA3

   Plasmid

   #80747

   Purpose

   Expression of human beta A3-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYBA3 (CRYBA1 Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC57 DYSF, alt exon 1, 5a, 40a

   Plasmid

   #87889

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a, 40a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. hME-pOX

   Plasmid

   #178181

   Purpose

   Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

   Depositor

   Ming-Feng Tsai

   Article

   Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

   Insert

   human MCU-EMRE fusion protein
   

   Use

   Tags

   1D4

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Spt2-GFP

   Plasmid

   #115572

   Purpose

   Expresses yeast Spt2-GFP fusion protein

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   SPT2
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4L59

   Plasmid

   #60087

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. L1-A426D

   Plasmid

   #15485

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-A426D (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 426 changed to Aspartic Acid

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pET28b-PU.1-ETS

   Plasmid

   #85734

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
   

   Use

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFP

   Plasmid

   #28011

   Depositor

   Lingchong You

   Article

   Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

   Insert

   CFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T-TRIM33-4

   Plasmid

   #166550

   Purpose

   scFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pID3.01B

    Plasmid

    #22720

    Depositor

    Geraldine Seydoux

    Article

    D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

    Insert

    unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

    Plasmid

    #91917

    Purpose

    Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LweCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYTK017

    Plasmid

    #65124

    Purpose

    Encodes pRPL18B as a Type 2 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    pRPL18B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_PLAT_Trypsin

    Plasmid

    #110039

    Purpose

    Protein expression and purification of PLAT_Trypsin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLAT_Trypsin (PLAT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAT221

    Plasmid

    #134889

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-TetOminiCMV-Klf4

    Plasmid

    #136613

    Purpose

    Dox-inducible lentiviral vector expressing mouse Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-APIGH

    Plasmid

    #112804

    Purpose

    gateway cloning destination vector to express enhancer driven Gal4 in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Neuron. 2012 Jan 12;73(1):64-78. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-HisD-mNG-C-Kbp

    Plasmid

    #178013

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-C-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-HisD-mNG-Pa-Kbp

    Plasmid

    #178014

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Pa-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-HisD-mNG-Hv-Kbp

    Plasmid

    #178015

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Hv-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge