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GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp7 C186A-His

Plasmid

#11826

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C186A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pJ2042.2

Plasmid

#140670

Purpose

3-color stripe

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJK503

Plasmid

#155380

Purpose

dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

May 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Tags

HA tag nuoN

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Tags

HA tag nuoG

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-06

Plasmid

#158434

Purpose

pBAD33 vector encoding MotA, Dendra2-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

Dendra2

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-03

Plasmid

#158433

Purpose

pBAD33 vector encoding MotA, yPET-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

yPET

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB3689

Plasmid

#47834

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 3 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pVRb03_up1198

Plasmid

#49706

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf03_up1198

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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TOPO UBC

Plasmid

#68456

Purpose

TOPO construct expressing UBC for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Ubiquitin C Promoter (UBC Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Expression

Bacterial

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-906)

Plasmid

#203333

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-906 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-35-H-6

Plasmid

#67008

Purpose

Expresses malE-35-H-6 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-35-H-6

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka-9-5

Plasmid

#104011

Purpose

Plasmid encoding Katushka-9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka9-5

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (K6S, N21D, R32G, L…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-APEX2

Plasmid

#117751

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

Depositor

Martin Spiess

Article

Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

Insert

anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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B5-4

Plasmid

#111483

Purpose

Trp-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Trp-tRNA synthetase

Tags

5xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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