30,307 results

1. pET51b-Strep-hAGT-His

   Plasmid

   #167276

   Purpose

   Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   hAGT (AGT Human)
   

   Use

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCellFree_G06_ZIKVC

   Plasmid

   #145349

   Purpose

   Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Capsid
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046940

   Plasmid

   #149261

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Human_TFAM_NoMTS_L6_pET28a+

   Plasmid

   #60012

   Purpose

   Expresses TFAM No MTS L6 mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT13araE

   Plasmid

   #18987

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   araE (araE E.coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pHAT

   Plasmid

   #112582

   Purpose

   Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ataxin3:1-431_Q79_BV

   Plasmid

   #209733

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   6xHis-SBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 13, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pXDC61-FabI

   Plasmid

   #21842

   Depositor

   Howard Shuman

   Article

   Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

   Insert

   fabI
   

   Use

   Tags

   TEM

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGMCZq17-OXOX

   Plasmid

   #31289

   Depositor

   Dirk Schnappinger

   Article

   Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

10. O-IL8-15

    Plasmid

    #166544

    Purpose

    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-PKM2-G415R

    Plasmid

    #122693

    Purpose

    Express His-tagged PKM2 (G415R) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    G415R

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

14. PIP5K2CA

    Plasmid

    #42352

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIP5K2CA (PIP4K2C Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-CARD14-G117S

    Plasmid

    #69920

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    G117S

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCph8

    Plasmid

    #50552

    Purpose

    Expresses Cph8 under a modified PLtetO-1 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    Cph8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLiP-G1

    Plasmid

    #188963

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQIq_MRGS_HIS8_( E3_5)_FLAG

    Plasmid

    #199612

    Purpose

    Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG E3_5 (control)
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pProEx-HTa-SOD1 C57D/C146D

    Plasmid

    #191851

    Purpose

    express C57D/C146D mutant SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C57D, C146D

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmNET

    Plasmid

    #35627

    Depositor

    Howard Gu

    Article

    Han et al BMC Pharmacol. 2006 Mar 3;6:6.

    Insert

    NET (Slc6a2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge