30,695 results

1. Let7-SunTag

   Plasmid

   #196928

   Purpose

   Let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Let7R-SunTag

   Plasmid

   #196929

   Purpose

   Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-parallel1-GK5_v2

   Plasmid

   #134288

   Purpose

   Expresses GK5 (isoform 2) in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   mouse Gk5 isoform 2; 534 amino acids

   Promoter

   T7

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVZ_PcoaT_SQE1_LUP1

   Plasmid

   #103857

   Purpose

   Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803

   Depositor

   Ilka Axmann

   Article

   Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

   Insert

   lupeol synthase 1 (LUP1 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   inserted genes are codon optimized. 2nd amino aci…

   Promoter

   PcoaT (from Synechocystis sp. PCC 6803)

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-24a-STE2

   Plasmid

   #133430

   Purpose

   Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   Both native Cys (C59S/C252A) have been mutated. S…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB32

   Plasmid

   #111911

   Purpose

   Expresses gRNA to target Nte1 locus and has HygR marker

   Depositor

   Yi Tang

   Insert

   gRNA cassette targeting Nte1 locus
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   SNR52p

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. RRAS1

   Plasmid

   #55659

   Purpose

   expression of mouse RRAS1

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RRAS1
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSM42

   Plasmid

   #113942

   Purpose

   Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

   Depositor

   Thomas Hughes, Robert Campbell

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jRGECO1a
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-HT_CtCHAD

   Plasmid

   #127785

   Purpose

   Expresses CtCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain)

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b_Qcat18

    Plasmid

    #45146

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT18
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Drp1(A395D)-StrepII

    Plasmid

    #174430

    Purpose

    Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    A395D

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Drp1-StrepII

    Plasmid

    #174428

    Purpose

    Expresses human Drp1 Isoform 3 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH016

    Plasmid

    #80400

    Purpose

    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. RAMP2

    Plasmid

    #39058

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RAMP2 (RAMP2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRF-AmeR

    Plasmid

    #49356

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    AmeR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    D120F, R121Q, G123H, Y124T, E125G, M126F, W127YS

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMALp2x-SCO5461

    Plasmid

    #42507

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pBbE6k-RFP

    Plasmid

    #35288

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-Pc4CL-CmCHS-MsCHI

    Plasmid

    #73398

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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