31,203 results
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Plasmid#168020PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cellsDepositorInsertBoNT/E-LC(1-411)-6xHis
TagsHisx6ExpressionBacterialAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJH027_SC101_pLAC_LbCas12a_Kan
Plasmid#183074PurposeWild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertLbCas12a
ExpressionBacterialMutationWild-typeAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSY6
Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMD5
Plasmid#138652PurposephiBT1 integrating Streptomyces expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRExpressionBacterial and PlantAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRExpressionBacterial and PlantPromoterU6 promoterAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZSm45 ndhII
Plasmid#48889PurposeLux-regulated expression of NDH-2 enzymeDepositorInsertndhII and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3(1-265)
Plasmid#100573PurposeExpresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.DepositorInsertStx3-1-265 (Stx3 Rat)
TagsGSTExpressionBacterialMutationNo transmembrane domain, residues 4-264 present.PromotertacAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
ExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRExpressionBacterialAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only