4,841 results

1. pAc5.1B-lambdaN-HA-DmG3BP_G

   Plasmid

   #146362

   Purpose

   Insect Expression of DmG3BP

   Depositor

   Elisa Izaurralde

   Insert

   DmG3BP (rin Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmGe1_1-558-V5His6_G

   Plasmid

   #146363

   Purpose

   Insect Expression of DmGe1_1-558

   Depositor

   Elisa Izaurralde

   Insert

   DmGe1_1-558 (Ge-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmGe1_559-943-V5His6_G

   Plasmid

   #146364

   Purpose

   Insect Expression of DmGe1_559-943

   Depositor

   Elisa Izaurralde

   Insert

   DmGe1_559-943 (Ge-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmImp8_G

   Plasmid

   #146365

   Purpose

   Insect Expression of DmImp8

   Depositor

   Elisa Izaurralde

   Insert

   DmImp8
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmLSm6_G

   Plasmid

   #146366

   Purpose

   Insect Expression of DmLSm6

   Depositor

   Elisa Izaurralde

   Insert

   DmLSm6 (CG9344 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmLSm8_G

   Plasmid

   #146367

   Purpose

   Insect Expression of DmLSm8

   Depositor

   Elisa Izaurralde

   Insert

   DmLSm8 (CG2021 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmUPF1-V5His6_E

   Plasmid

   #146165

   Purpose

   Insect Expression of DmUPF1

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1 (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two non silent mutations E531G and G995E compared…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-DmUPF3-siRNAres-V5His6_E

   Plasmid

   #146167

   Purpose

   Insect Expression of DmUPF3-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3-siRNAres (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and one non silent mutation P305S compar…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmUPF1-siRNAres-V5His6_E

   Plasmid

   #146168

   Purpose

   Insect Expression of DmUPF1-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1-siRNAres (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   1 non silent mutations: G995E compared to the seq…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmUPF2-siRNAres-V5His6_E

    Plasmid

    #146169

    Purpose

    Insect Expression of DmUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF2-siRNAres (Upf2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent and 3 non silent mutations: L1022P, V10…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OA-1085L

    Plasmid

    #191376

    Purpose

    pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OA-1093B

    Plasmid

    #194003

    Purpose

    pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting CHIKV
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OA-1163A

    Plasmid

    #194001

    Purpose

    pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    Uniq-CasRx (Codon optimized)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OA-1050T

    Plasmid

    #191374

    Purpose

    pBac-AedesUbiq-RfxCas13d-p10-Opie2-dsRed.

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    Uniq-CasRx
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OA-1085F

    Plasmid

    #191375

    Purpose

    pBac-U6-crYellow-array-UTR-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting yellow
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmGe1_J

    Plasmid

    #146688

    Purpose

    Insect Expression of DmGe1

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    DmGe1 (Ge-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two non silent mutations Q194R and I1008T compare…

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-DmAdhV5-5BoxB_X

    Plasmid

    #147910

    Purpose

    Insect Expression of DmAdh-5BoxB

    Depositor

    Elisa Izaurralde

    Insert

    DmAdh-5BoxB (Adh Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CapChR1_pUAST-attb

    Plasmid

    #188040

    Purpose

    Expresses CapChR1 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1
    

    Use

    Tags

    none

    Expression

    Insect

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    UAS

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Chinchilla–insM

    Plasmid

    #191226

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed methionine at 122 in the protein sequence

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Chinchilla+N122D–insM

    Plasmid

    #191227

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed methionine at 122 and replaced asparagine…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Sandgrouse–insD

    Plasmid

    #191228

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed aspartic acid at 121 in the protein seque…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Sandgrouse+R111Q–insD

    Plasmid

    #191229

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed aspartic acid at 121 and replaced arginin…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UAS-GFP::NMN-Deamidase

    Plasmid

    #187868

    Purpose

    Gal4/UAS expression of GFP::NMN-Deamidase

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    NMN-Deamidase
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOPINF Ta-sro1 PARP

    Plasmid

    #192549

    Purpose

    E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
    

    Use

    Tags

    His6, 3C protease cleavage site

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5-STABLE2-neo-RvCAHS3

    Plasmid

    #192472

    Purpose

    Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    T2A-EGFP-T2A-neoR

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

    Plasmid

    #192470

    Purpose

    Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

    Plasmid

    #192469

    Purpose

    Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

    Plasmid

    #192705

    Purpose

    Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728

    Depositor

    Sascha Martens

    Inserts

    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16L1
    

    Use

    Tags

    -GFP-TEV-StrepII and 10x-His-TEV

    Expression

    Insect

    Mutation

    F32A-I35A-I36A

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAFMW-Nbr[R272A,K276A, R280A,K284A]

    Plasmid

    #188599

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge