4,841 results

1. pFastBac1-Clover4-LUMABS-S1 RBD

   Plasmid

   #183491

   Purpose

   To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

   Depositor

   Jun Chu, Zongwen Jin

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-S1 RBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor_C_FH_Nbr

   Plasmid

   #186662

   Purpose

   Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nbr_C_sgRNA2

   Plasmid

   #186664

   Purpose

   Nbr C-tag sgRNA2 plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Nbr sgRNA 2 Plasmid (Nbr Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nbr_C_sgRNA1

   Plasmid

   #186663

   Purpose

   Nbr C-tag sgRNA1 plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Nbr sgRNA 1 Plasmid (Nbr Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Shu_sgRNA

   Plasmid

   #186656

   Purpose

   shu sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Shu sgRNA Plasmid (shu Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9_nonPuro_OSS

   Plasmid

   #186652

   Purpose

   Cas9 plasmid to express the Cas9 in OSS cells

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Cas9 Expression Plasmid
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. puc19_N_tag

   Plasmid

   #186650

   Purpose

   Empty donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   N-terminal 3xFlag-3xHA Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDonor_N_FH_GLE

   Plasmid

   #186657

   Purpose

   Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VIR_sgRNA

   Plasmid

   #186661

   Purpose

   VIR sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   VIR sgRNA Plasmid (vir Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid

    #185448

    Purpose

    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FAK Fus

    Plasmid

    #186145

    Purpose

    For expression of recombinant FAK using baculovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with FUS IDR

    Promoter

    polyhedrin promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac-M13-6His-KIF1C_P176L-GFP

    Plasmid

    #185978

    Purpose

    Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lck

    Plasmid

    #186130

    Purpose

    For expression of recombinant Lck in SF9 cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    Lck (Lck Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    A1687T

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FAK Pyk2

    Plasmid

    #186146

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with Pyk2 linker

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BMP10 N67Q N131Q D4G (S2-2)

    Plasmid

    #184622

    Purpose

    for transfection into S2 cells and expression of BMP10 protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    BMP10 (BMP10 Human)
    

    Use

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Mutation

    N67Q, N131Q, ARIRR(312-316) replaced with G

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1056D

    Plasmid

    #183132

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1056E

    Plasmid

    #183133

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[dsx]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1056F

    Plasmid

    #183134

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[tra]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1056H

    Plasmid

    #183135

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 1056J

    Plasmid

    #183136

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH

    Plasmid

    #148935

    Purpose

    Insect Expression of DmGIGYF_340-368

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_340-368 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSN526

    Plasmid

    #184827

    Purpose

    Expresses human KIF5A(∆exon27) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Use

    Tags

    mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    polyhedrin

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-EGFP-DmCaf40-A253FY208A_X

    Plasmid

    #147973

    Purpose

    Insect Expression of DmCaf40-A253FY208A

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmCaf40-A253FY208A (Rcd-1 Fly)
    

    Use

    Tags

    EGFP (A179V)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

    Plasmid

    #148160

    Purpose

    Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    alias: 4E-T; one non silent mutation L127S, annot…

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-EGFP-HsTNRC6C_L

    Plasmid

    #146855

    Purpose

    Insect Expression of HsTNRC6C

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsTNRC6C (TNRC6C Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation Q1183K compard to the seq…

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ppBAalbNixE1E3E4 PUb-YFP

    Plasmid

    #173666

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    YFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Insect

    Mutation

    Nix isoforms with exons E1, E3, E4

    Promoter

    Aedes aegypti PUb and own

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-EGFP-HsTNRC6A_L

    Plasmid

    #146843

    Purpose

    Insect Expression of HsTNRC6A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsTNRC6A (TNRC6A Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    A339T, K1578E compared to the sequence of isoform…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

    Plasmid

    #147580

    Purpose

    Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge