4,841 results

1. pAc5.1C-Fluc-nerfin_E

   Plasmid

   #146190

   Purpose

   Insect Expression of nerfin-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

   Insert

   nerfin-3UTR (nerfin-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

   Plasmid

   #146166

   Purpose

   Insect Expression of DmUPF2-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF2-siRNAres (Upf2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   3 non silent mutations: L1022P, V1097L and N1213S…

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFL GST-UBXN2A

   Plasmid

   #169016

   Purpose

   Expresses GST-UBXN2A (human) in insect cells

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN2A (UBXN2A Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAC-U63-tgRNA-Gal80

   Plasmid

   #169030

   Purpose

   gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

   Depositor

   Chun Han

   Article

   Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

   Plasmid

   #146071

   Purpose

   Insect Expression of DmTral-LSmR21EE23KR42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KR42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R21E, E23K, R42E, F44A. Additionally, one silent …

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmTral-R42EF44A_C

   Plasmid

   #146061

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R42E, F44A. Additionally, two silent mutations A1…

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACEBac1-Q22YU3 del C3-2xStrep

   Plasmid

   #170316

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 C-terminal truncation (Delta-C3)
   

   Use

   Tags

   Strep-Tag II

   Expression

   Insect

   Mutation

   Deletion of C-terminal C3-finger

   Promoter

   polyhedrin

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACEBac1-Q22MS1-2xStrep

    Plasmid

    #170314

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D

    Plasmid

    #146156

    Purpose

    Insect Expression of 5BoxB-7-Fluc-CG10011-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    5BoxB-7-Fluc-CG10011-3UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmMe31B_268-459_D

    Plasmid

    #146119

    Purpose

    Insect Expression of DmMe31B_268-459

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmMe31B_268-459 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-DmPABPC1-V5His6_D

    Plasmid

    #146095

    Purpose

    Insect Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    seven silent mutations compared to the sequence g…

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVMG0193_Cq-Rpl40-Cas9

    Plasmid

    #169346

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0193_Cq-Rpl40-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ002413

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVMG0217_Cq-U6-2b_2xBbsI-gRNA

    Plasmid

    #169339

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0217_Cq-U6-2b_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039728

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

    Plasmid

    #146013

    Purpose

    Insect Expression of DmSmaug_763-999

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_763-999 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    2 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmTral-R42E_B

    Plasmid

    #145988

    Purpose

    Insect Expression of DmTral-R42E

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42E (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations A198C, A387G and a mutation …

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

    Plasmid

    #146001

    Purpose

    Insect Expression of DmSmaug_1-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_1-762 (smg Fly)
    

    Use

    Tags

    V5-6xHis

    Expression

    Insect

    Mutation

    NP_523987.1, 7 silent mutations compared to the s…

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmZuc_B

    Plasmid

    #145949

    Purpose

    Insect Expression of DmZuc

    Depositor

    Elisa Izaurralde

    Insert

    DmZuc (zuc Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmExu_B

    Plasmid

    #145939

    Purpose

    Insect Expression of DmExu

    Depositor

    Elisa Izaurralde

    Insert

    DmExu (exu Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

    Plasmid

    #145898

    Purpose

    Insect Expression of DmAGO1-F629V

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-F629V (AGO1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V315A, F629V, H913R - variation with 2 non silent…

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-HA-DmLSm3_A

    Plasmid

    #145888

    Purpose

    Insect Expression of DmLSm3

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmLSm3 (LSm3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-EGFP-DmDCP2_209-791_A

    Plasmid

    #145848

    Purpose

    Insect Expression of DmDCP2_209-791

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2_209-791 (DCP2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac1_GI.1_A37C-A44C

    Plasmid

    #162582

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Alanine 37 changed to Cysteine and Alanine 44 cha…

    Promoter

    polyhedring

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac1_GI.1_A37I-A44L

    Plasmid

    #162583

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    A37I and A44L

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBac1_GI.1_Q141V-P221L

    Plasmid

    #162584

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glutamine 141 changed to Valine and Proline 221 c…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBac1_GI.1_L144C-P221C

    Plasmid

    #162585

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Leucine 144 changed to Cysteine and Proline 221 c…

    Promoter

    polyhderin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac1_GI.1_G131C-N172C

    Plasmid

    #162586

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glycine 131 changed to Cysteine and Asparagine 17…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac1_GI.1_N167C-L169C

    Plasmid

    #162587

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 167 changed to Cysteine and Leucine 16…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge