4,840 results

1. pACEBac1_Strep-Psc-BICD2(711-800)_L742A

   Plasmid

   #228839

   Purpose

   Insect cell expression of the mutant C-terminus of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800, with L742A

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac SOX R139A

   Plasmid

   #192058

   Purpose

   Expression vector for SOX (ORF37) R139A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R139A

   Promoter

   Polyhedrin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac SOX 318-320A

   Plasmid

   #192060

   Purpose

   Expression vector for SOX (ORF37) 318-320A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   318-320A

   Promoter

   Polyhedrin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac WT SOX

   Plasmid

   #192055

   Purpose

   Expression vector for WT SOX

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   WT SOX (ORF37)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac SOX Q129H

   Plasmid

   #192057

   Purpose

   Expression vector for SOX (ORF37) Q129H

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37) Q129H
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Q129H

   Promoter

   Polyhedrin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUASTattB-degradALFA

   Plasmid

   #219663

   Purpose

   Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   degradALFA
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

   Plasmid

   #226884

   Purpose

   Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

   Depositor

   Ning Zheng

   Article

   Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

   Insert

   NaVAb
   

   Use

   Tags

   MBP

   Expression

   Insect

   Mutation

   R4A, N49K, L109A, M116V

   Promoter

   Polyhedrin

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

   Plasmid

   #222300

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[451-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

   Plasmid

   #222301

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[571-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBIG1a_StrepII-Hook2_FTS_FHIP2A

    Plasmid

    #222302

    Purpose

    Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

    Depositor

    Andrew Carter

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Inserts

    HOOK2 (HOOK2 Human)
    FTS (AKTIP Human)
    FHIP2A (FHIP2A Human)
    

    Use

    Tags

    StrepII-Psc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac1-NEDD4L_ΔC2 (157)

    Plasmid

    #187720

    Purpose

    Express GST-tagged NEDD4L ΔC2 (157) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 157-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-156

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

    Plasmid

    #187721

    Purpose

    Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 349-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-348

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBacHTA-NEDD4L_FL

    Plasmid

    #187722

    Purpose

    Express His-tagged NEDD4L FL in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

    Plasmid

    #187723

    Purpose

    Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 1-418+582-975 (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    deletion of residues 419-581

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBacHTA-NEDD4L_W1-L-W2-HECT

    Plasmid

    #187724

    Purpose

    Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 153-418+582-975 (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    deletion of residues 1-152 and 419-581

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Dm_λHA-FLuc100_CSC

    Plasmid

    #210592

    Purpose

    Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)
    

    Use

    Tags

    lambda HA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dm_Opt_Renilla_CSC

    Plasmid

    #210593

    Purpose

    Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Dm_λHA-FLuc0_CSC

    Plasmid

    #210589

    Purpose

    Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
    

    Use

    Luciferase

    Tags

    lambda HA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacI-KDAC7 CD

    Plasmid

    #224345

    Purpose

    Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC7 (HDAC7 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Only includes residues 521-942 (catalytic domain).

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFastBacI-KDAC6 H216A

    Plasmid

    #224351

    Purpose

    Expresses human KDAC6 (HDAC6) H216A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 216 mutated to alanine

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pFastBacI-KDAC6 H611A

    Plasmid

    #224352

    Purpose

    Expresses human KDAC6 (HDAC6) H611A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 611 mutated to alanine

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pKC52

    Plasmid

    #226780

    Purpose

    Expresses Drosophila UNC-104(R348G) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R348G) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 348 to Glycine

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKC50

    Plasmid

    #226778

    Purpose

    Expresses Drosophila UNC-104(V6M) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (V6M) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Valine 6 to Methionine

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKC28

    Plasmid

    #226776

    Purpose

    Expresses Drosophila UNC-104(G618R) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (G618R) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    G618R

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKC31

    Plasmid

    #226777

    Purpose

    Expresses Drosophila UNC-104(R562H) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R562H) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 562 to Histidine (bris mutation)

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSN539

    Plasmid

    #226775

    Purpose

    Expresses Drosophila UNC-104(wild-type) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKC51

    Plasmid

    #226779

    Purpose

    Expresses Drosophila UNC-104(A255V) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (A255V) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Alanine 255 to Valine

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFBOH-MHL.mEGFP-CBX8

    Plasmid

    #224710

    Purpose

    Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge