Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

4,958 results

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pACUH 5-HT7R-7xGFP11-HA

Plasmid

#221836

Purpose

Plasmid for generating 10xUAS-5-HT7R-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT7R (5-HT7 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3

Plasmid

#240233

Purpose

Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

Myc-BirA*G3

Expression

Insect

Promoter

MT

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-23012

Plasmid

#109180

Purpose

Plasmid for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-23122

Plasmid

#109181

Purpose

Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23003

Plasmid

#109183

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23004

Plasmid

#109184

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacD-23005

Plasmid

#109185

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21222-SNF5

Plasmid

#109186

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged SNF5 under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Insert

SNF5 (SMARCB1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21122

Plasmid

#109174

Purpose

Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21222

Plasmid

#109175

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21232

Plasmid

#109177

Purpose

Plasmid for expression of C-terminally CBP-3xFLAG-ProteinA-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

CBP-3xFLAG-ProteinA

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG-BAF57

Plasmid

#109189

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 and untagged BAF57 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Inserts

BRG1 (SMARCA4 Human)
BAF57 (SMARCE1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-RBAP46/RBBP7

Plasmid

#109210

Purpose

Encodes human full-length RBBP7 (aka RBAP46) to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length RBBP7 (RBBP7 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-SAP30

Plasmid

#109212

Purpose

Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

Human full-length SAP30 (SAP30 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21222-SIN3A-3xFLAG

Plasmid

#109213

Purpose

Encodes human full-length SIN3A with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3A (SIN3A Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21222-SIN3B-3xFLAG

Plasmid

#109214

Purpose

Encodes human full-length SIN3B with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3B (SIN3B Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-HDAC1

Plasmid

#109209

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human, Synthetic)

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-20XUAS-IVS-Syn21-Positron2-p10

Plasmid

#239082

Purpose

Gal4 activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Insect

Mutation

R78K N81D D92N W178F

Promoter

hsp70

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10

Plasmid

#239081

Purpose

LexA activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Insect

Mutation

R78K N81D D92N W178F

Promoter

hsp70

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Plasmid

#238314

Purpose

ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

Plasmid

#238327

Purpose

ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E576A-K623A-Q625S-K627A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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