4,841 results
-
Plasmid#219987PurposeDDX3X(G325E) protein purificationDepositorInsertDDX3X(G325E) (DDX3X Human)
UseTagsMBP and monoGFP and 6xHisExpressionInsectMutationG325EPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC120-MBP-Ded1-monoGFP
Plasmid#219983PurposeDed1p-monoGFP protein purificationDepositorInsertDed1p-monoGFP (DED1 Budding Yeast)
UseTagsMBP and monoGFP-6xHisExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-SkA
Plasmid#219977PurposeeIF4G-SkA protein purificationDepositorInserteIF4G-SkA (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutationeIF4A binding domain of S. kudriavzeviiPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-213
Plasmid#219974PurposeeIF4G-213 protein purificationDepositorInserteIF4G-213 (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutation213VKLR216 to 213AAAA216PromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC121-MBP-monoGFP-eIF4G
Plasmid#219972PurposeeIF4G protein purificationDepositorInserteIF4G (TIF4631 Budding Yeast)
UseTags6xHis and MBP-monoGFPExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-RAR
Plasmid#219973PurposeeIF4G-RAR protein purificationDepositorInserteIF4G-RAR (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutationTruncation: amino acids 490 to 942PromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
Plasmid#217514PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt1
Plasmid#220239PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationR27A, R31A, F165APromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
Plasmid#217509PurposeKD Recombinase dependent conditional 1X ALFA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
UseTagsALFA tagExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
1167A
Plasmid#218233PurposeHDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae geneDepositorInsertputative metabolite transport protein
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hTspan12LEL(118-218)_pAcGP67a
Plasmid#216383PurposeBaculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBPDepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3CExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
I14-pAc5.1 lambda N HA Cup
Plasmid#206493PurposeCup expression for Schneider cell imagingDepositorTypeEmpty backboneUseTagslambda N HAExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
F23-pAc5.1 EGFP Cup
Plasmid#206488PurposeCup expression for Schneider cell imagingDepositorInsertCup
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
F17-pAc5.1 EGFP Vasa 1-200
Plasmid#206484Purposevasa 1-200 expression for Schneider cell imagingDepositorInsertVasa 1-200
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_052
Plasmid#216082Purposefor stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion, destination vectorDepositorHas ServiceCloning Grade DNAInsertHygromycin resistance gene
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_051
Plasmid#216081Purposefor stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertattL and attR sequences for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_MAGEA3:104-314
Plasmid#210941PurposeInsect expression of human MAGEA3 fragment E104 to E314. Untagged.DepositorInsertMAGEA3:E104-E314 (MAGEA3 Human)
UseTagsExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_EIF2A:1-427
Plasmid#210939PurposeInsect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF2A:M1-T427 (EIF2A Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_UTP15:1-370
Plasmid#210937PurposeInsect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.DepositorInsertUTP15:M1-H370 (UTP15 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_TBL2:70-405
Plasmid#210936PurposeInsect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.DepositorInsertTBL2:E70-H405 (TBL2 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_SHKBP1:182-585
Plasmid#210935PurposeInsect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.DepositorInsertSHKBP1:T182-L585 (SHKBP1 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationQ507L natural variant (VAR_036714)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_IFT172:1-320
Plasmid#210934PurposeInsect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.DepositorInsertIFT172:M1-S320 (IFT172 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_WDR19:1-636
Plasmid#210933PurposeInsect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR19:M1-G636 (WDR19 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_WDR73:1-378
Plasmid#210932PurposeInsect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR73:M1-R378 (WDR73 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits