4,958 results
-
Plasmid#206491PurposeOskar 139-606 expression for Schneider cell imagingDepositorInsertOskar 139-606
TagsmCherryExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F16-pAc5.1 EGFP Vasa closed
Plasmid#206487PurposeVasa (closed conformation) expression for Schneider cell imagingDepositorInsertvasa E400Q
TagsEGFPExpressionInsectMutationE400QAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F19-pAc5.1 EGFP Vasa 463-661
Plasmid#206486PurposeVasa 463-661 expression for Schneider cell imagingDepositorInsertVasa 463-661
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F18-pAc5.1 EGFP Vasa 200-462
Plasmid#206485PurposeVasa 200-642 expression for Schneider cell imagingDepositorInsertVasa 200-462
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F15-pAc5.1 EGFP Vasa
Plasmid#206483PurposeVasa expression for Schneider cell imagingDepositorInsertVasa
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F20-pAc5.1 EGFP Aubergine
Plasmid#206482PurposeAubergine expression for Schneider cell imagingDepositorInsertAubergine
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
ExpressionInsectAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASt-Xbp1-HA-GFP-3'UTR Spl
Plasmid#201246PurposeXbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced formDepositorInsertdXbp1 (Xbp1 Fly)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST ɑSynWT-EGFP
Plasmid#201249Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertɑ-Synuclein (SNCA Human)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-JEDI-1P
Plasmid#202618PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expressionDepositorInsertJEDI-1P
ExpressionInsectPromoterhsp70Available SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TEG +Q111T
Plasmid#187435PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationchanged glutamine at 117 to threoninePromoterPH and p10Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS WT-HA-GFP
Plasmid#199687PurposeExpression of LYCHOS WT in insect cellsDepositorAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
IR76b-T2A-QF2
Plasmid#162523PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b geneDepositorInsertIr76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
ExpressionInsectAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
GR3-T2A-QF2
Plasmid#162521PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 geneDepositorInsertGr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
ExpressionInsectAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
IR8a-T2A-QF2
Plasmid#162520PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a geneDepositorInsertIr8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
ExpressionInsectAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS FP>IA-HA-GFP
Plasmid#199688PurposeExpression of LYCHOS FP>IA mutant in insect cellsDepositorInsertLYCHOS (GPR155 Human)
Tags10xHis, 6xHis, EGFP, HA, and HRV 3CExpressionInsectMutationF43 and P44 mutated to I and A respectivelyAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…ExpressionInsectPromoterU6-3Available SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)
Plasmid#198072PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptAD-seq-ubi63E-Gal4-DBD
Plasmid#111930PurposeVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.DepositorInserttAD-seq Gal4-DBD
ExpressionInsectAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26_4xUAS_DSCP_GFP
Plasmid#111931PurposeVector to express GFP (under the control of the DSCP and an array of 4 UAS sites)DepositorInsert4xUAS GFP
ExpressionInsectAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_1-640_AH
Plasmid#148933PurposeInsect Expression of DmGIGYF1_1-640DepositorInsertDmGIGYF1_1-640 (Gyf Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH
Plasmid#148934PurposeInsect Expression of DmGIGYF_331-374DepositorInsertDmGIGYF_331-374 (Gyf Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH
Plasmid#148936PurposeInsect Expression of DmGIGYF1_641-1574DepositorInsertDmGIGYF1_641-1574 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only