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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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80,205 results

Showing: 11261 - 11280 of 80205 results

  4. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  7. CNR2-DuET

    Plasmid
    #213214
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CNR2 (CNR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213214-plasmid-map-sequence-id-430776

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  11. MRGPRX4-Tango

    Plasmid
    #66442
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX4 (MRGPRX4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1-hNurr1-NE

    Plasmid
    #102363
    Purpose
    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells
    Depositor
    Shu Leong Ho
    Insert
    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    Tags
    NE
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102363-plasmid-map-sequence-id-200857

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Showing: 11261 - 11280 of 80205 results