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LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ

Plasmid

#192383

Purpose

To act as the off state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

Plasmid

#192384

Purpose

To act as the on state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ

Plasmid

#192416

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ

Plasmid

#192417

Purpose

To act as the 2-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ

Plasmid

#192415

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1

Plasmid

#192414

Purpose

To act as the 2-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ

Plasmid

#192408

Purpose

To act as a 1-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

Plasmid

#192379

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge