4,298 results
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Plasmid#207510PurposeePPE for prime editing in riceDepositorInsertzCas9 (H840A)-NC-M-MLV RT-deltaRH
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL682
Plasmid#205591PurposePE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL683
Plasmid#205593PurposeCas9-PE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E7
Plasmid#213470PurposeGateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cdd-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E8
Plasmid#213471PurposeGateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cdd_v106w-32aa li…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL854
Plasmid#214254PurposeExpression eTd-CBE in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGM13451
Plasmid#219522PurposeTransient expression of At BGLU10DepositorInsertAt BGLU10
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM19477
Plasmid#219521PurposeTransient expression of At SATDepositorInsertAt SAT
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM19465
Plasmid#219520PurposeTransient expression of At 79B1DepositorInsertAt UGT79B1
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM13437
Plasmid#219519PurposeTransient expression of At 5MATDepositorInsertAt 5MAT
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM13425
Plasmid#219518PurposeTransient expression of At 5GTDepositorInsertAt 5GT
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM13414
Plasmid#219517PurposeTransient expression of At 3AT1DepositorInsertAt 3AT1
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM81987
Plasmid#219516PurposeSilencing of Nb RTDepositorInsertNb RT
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLC045 - pL0_CrGLK (CDS1ns)
Plasmid#203904PurposeMoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.DepositorInsertCrGLK (without a stop codon)
UseTagsExpressionPlantMutationSilent mutation of BpiI or BsaI cut site for MoCl…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC047 - pL2_p2x35s-CrGLK-tMAS
Plasmid#203906PurposeOverexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.DepositorInsertCrGLK
UseTagsExpressionPlantMutationSilent mutation of BpiI or BsaI cut site for MoCl…PromoterCaMV 2x35SAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN381 - pTRV2-CHLH
Plasmid#203886PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.DepositorInsertCHLH fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN378 - pTRV2-CrGLK
Plasmid#203888PurposeViral silencing plasmid targeting the CrGLK gene from C. roseus.DepositorInsertCrGLK fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB278 - pL0_pT16H2 (pro + 5U)
Plasmid#203889PurposeT16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT16H2 promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1213 - pL0_p16OMT (pro + 5U)
Plasmid#203890Purpose16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsert16OMT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1243 - pL0_pT3O (pro + 5U)
Plasmid#203891PurposeT3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT3O promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1224 - pL0_pT3R (pro + 5U)
Plasmid#203892PurposeT3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT3R promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1214 - pL0_pNMT (pro + 5U)
Plasmid#203893PurposeNMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertNMT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1198 - pL0_pD4H (pro + 5U)
Plasmid#203894PurposeD4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertD4H promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1215 - pL0_pDAT (pro + 5U)
Plasmid#203895PurposeDAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertDAT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB55 - pL0_CrGLK (CDS)
Plasmid#203903PurposeMoClo compatible L0 plasmid containing the CrGLK coding sequenceDepositorInsertCrGLK
UseTagsExpressionPlantMutationSilent mutation of BpiI or BsaI cut site for MoCl…PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1139T8C
Plasmid#210837PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1131T10C
Plasmid#210836PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1131T8C
Plasmid#210835PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-OEP7-mVenus
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-po-mCerulean
Plasmid#213861PurposeBinary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)DepositorInsertPeroxisome transit peptide fused mCerulean
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZG162
Plasmid#202581PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P63
Plasmid#195938PurposePhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35SDepositorInsertpUBQ10-PI-tUBQ1-p35S-IP-t35S
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P27
Plasmid#195929PurposeLevel 1 PhiC31 cassette with constitutive promoter: p35S-UbArg-PhiC31NLS-tUBQ1DepositorInsertp35S-UbArg-PhiC31NLS-tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT2
Plasmid#210550PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
UseTagsExpressionPlantMutation1bp insertion after AAVS1 target sequence that di…PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipYUC2Cas-LT1
Plasmid#210559PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.DepositorInsertpMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1Cas-LT1
Plasmid#210556PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1pDepositorInsertpMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[]:mNeonGreen-I{PIV-198}:NosT
Plasmid#212165PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoterDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB605-GBSSI
Plasmid#213867PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB560-GBSSI
Plasmid#213866PurposeBinary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:nnLuz-I{PIV-216}:NosT
Plasmid#212185PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[TMV]:nnLuz-I{PIV-216}:NosT
Plasmid#212186PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the original 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promoteroriginal 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:nnLuz-I{PIV-216}:NosT
Plasmid#212187PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoterDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:nnLuz:NosT
Plasmid#212188PurposeThis binary vector expresses mushroom luciferase (nnLuz) with the truncated 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz
UseTagsExpressionPlantMutationPromotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[TMV]:nnLuz:NosT
Plasmid#212189PurposeThis binary vector expresses mushroom luciferase (nnLuz) with the original 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz
UseTagsExpressionPlantMutationPromoteroriginal 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT
Plasmid#212170PurposeThis binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PDK-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PDK int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:mNeonGreen-CI{PIV-198}:NosT
Plasmid#212171PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoterDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-198}:NosT
Plasmid#212172PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only