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pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

Plasmid

#182347

Purpose

GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBN303

Plasmid

#86730

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Punc-47::FLP D5

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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SREC 2: abu-1::abu-1::gfp

Plasmid

#21825

Depositor

David Ron

Article

Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

Insert

abu-1 promoter and coding region (abu-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMLS236

Plasmid

#73684

Purpose

SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJRM3 - [BAGp | mScarlet | tbb-2 UTR]

Plasmid

#204616

Purpose

mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | wrmScarlet | tbb-2 UTR]

Expression

Worm

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJRM4 - [BAGp | gfp | tbb-2 UTR]

Plasmid

#204617

Purpose

gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | gfp | tbb-2 UTR]

Expression

Worm

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159869

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCM3.19

Plasmid

#19401

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

tbb-2 ORF+3' in pDONR201 (tbb-2 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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TU#713

Plasmid

#15281

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czyfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1121

Plasmid

#78188

Purpose

intestinal GFP expression by fasting in worms

Depositor

Junho Lee

Article

Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

Insert

fil-2 (fil-2 Nematode)

Expression

Worm

Promoter

fil-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pRB1081

Plasmid

#71479

Purpose

Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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L2534

Plasmid

#1608

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, IVSA, rf2128, IVSM, IVSL, dec…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L4271

Plasmid

#1646

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T3p, gfpN, gfpS65S, gfpC, unc2217896, unc223827, …

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pJH4260

Plasmid

#191678

Purpose

Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP6s

Tags

wScarlet

Expression

Worm

Promoter

Ptwk-40

Available Since

Nov. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pPD158.87

Plasmid

#1709

Depositor

Andrew Fire

Type

Empty backbone

Tags

sur5-10k, sur5tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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