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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH51

Plasmid

#139045

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Expression

Worm

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDD316

Plasmid

#137020

Purpose

mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Insert

mTurq2-GLO^SEC^2xHA + ccdB

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans GLO-optimized mTurq2

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH50

Plasmid

#139037

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSM05

Plasmid

#139050

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJF7

Plasmid

#139036

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (no STOP codon) (tomm-20 Nematode)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH66

Plasmid

#139053

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

unc-54 3'UTR (unc-54 Nematode)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY847

Plasmid

#135093

Purpose

Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

Depositor

Yishi Jin

Article

Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

Insert

Pacr-2

Tags

mcherry and unc-10 3'UTR

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJH2256

Plasmid

#132365

Purpose

Prgef-1 INS-6::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-6::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-6 (ins-6 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Jan. 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2933

Plasmid

#132363

Purpose

Prgef-1 INS-3::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-3::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-3 (ins-3 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Dec. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJH1922

Plasmid

#132361

Purpose

Prgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-1::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-1 (ins-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2255

Plasmid

#132364

Purpose

Prgef-1 INS-4::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-4::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-4 (ins-4 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2947

Plasmid

#132362

Purpose

Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-2 (ins-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

Plasmid

#129539

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TC562

Plasmid

#122618

Purpose

Punc-4::rtetR-QFAD(650-777+790-816)

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD(650-777+790-816)

Expression

Worm

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::tagBFP

Plasmid

#124343

Purpose

Nuclear Localized Pan-Neuronal tagBFP

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, tagBFP (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::mCherry

Plasmid

#124348

Purpose

Nuclear Localized Pan-Neuronal mCherry

Depositor

Andrew Leifer

Insert

rab-3, mCherry (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-tax-4P::his-24::mOrange

Plasmid

#124340

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

tax-4, mOrange (tax-4 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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