1,934 results

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pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA844 - hrp-1p::hrp-1mScarlet

Plasmid

#139204

Purpose

Expresses mScarlet tagged hrp-1

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA852 - mec-4p::FynY531F

Plasmid

#139209

Purpose

Expresses activated Fyn kinase in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Plasmid

#159838

Purpose

Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Expression

Worm

Mutation

Not applicable

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Publication

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1884

Plasmid

#154332

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1948

Plasmid

#154340

Purpose

Enhancer reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDD289

Plasmid

#137018

Purpose

GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Insert

GFP-GLO^SEC^3xFlag + ccdB

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans GLO-optimized GFP

Expression

Worm

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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