8,432 results
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Plasmid#67535PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCfB2193
Plasmid#67542PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorPublicationInsertNum1-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorPublicationInsertOsh1-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LD116
Plasmid#69168PurposeIntegration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg16-GFP
TagsEGFPExpressionYeastAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD229
Plasmid#69192PurposeIntegration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg29-2EGFP
Tags2xEGFPExpressionYeastAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD221
Plasmid#69189PurposeIntegration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg21-2EGFP
Tags2xEGFPExpressionYeastAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38
Plasmid#65516PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edCit-attR1 and attR2-edCer-cMycExpressionYeastPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG211
Plasmid#64519PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP34
Plasmid#65512PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionYeastPromoterpPMA1Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK096
Plasmid#65203PurposePre-Assembled Ura3 integration vector to be used in the Dueber YTK system.DepositorPublicationTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
ExpressionYeastMutationValine 163 changed to alanineAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYC61
Plasmid#63925PurposeRecipient replicative vector to construct CFP tagged fusionsDepositorTypeEmpty backboneTagsCFPExpressionYeastAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC55
Plasmid#63922PurposeRecipient replicative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneTagsYFPExpressionYeastAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC42
Plasmid#63920PurposeRecipient replicative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
TagsFLAGExpressionYeastPromoterGAL10Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2
Plasmid#60388PurposeExpression of yeast beta tubulin (TUB2) using a GAL promoterDepositorInsertTUB2
ExpressionYeastPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-MYO1
Plasmid#51570PurposeConstitutively express myosin ring localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsMYO1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by