8,390 results
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Plasmid#58559PurposeMulticopy vector with Hygromycin resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only
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M4753 KanMX::ADE2 Disruptor Converter
Plasmid#51683PurposeYeast marker swap plasmid for converting KanMX3 to ADE2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
D1433 his3::LYS2 Disruptor Converter
Plasmid#51676PurposeYeast marker swap plasmid for converting HIS3 to LYS2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
M3938 trp1::ADE2 Disruptor Converter
Plasmid#51673PurposeYeast marker swap plasmid for converting TRP1 to ADE2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
D588 lys2::HIS3 Disruptor Converter
Plasmid#51675PurposeYeast marker swap plasmid for converting LYS2 to HIS3DepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDBD-M
Plasmid#50572PurposeDouble Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571DepositorInsertDHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
ExpressionYeastPromoterGal1Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHDV
Plasmid#51058PurposeSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHH
Plasmid#51057PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYEXNHG3
Plasmid#49025PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterGAL1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMC6-TEFkFLP(LYS2)
Plasmid#49458Purposeoverespression of Flp step-arrest mutant in yeast, LYS2 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
ExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterTEF1Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH001
Plasmid#47953PurposetetO-GFP reporter casetteDepositorInsertGFP
UseTet inducible expressionExpressionYeastPromoterT3Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAH2
Plasmid#41826PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7, TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG18-GFP
Plasmid#42529DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG17-GFP
Plasmid#44175DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
YCplac111-yVps23-mCherry
Plasmid#45925DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16
Plasmid#45124DepositorInsertDnvLB16
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-CaURA3
Plasmid#44879PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selectionDepositorInsertyomApple
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-SpHis5
Plasmid#44847PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selectionDepositorInsertyomKeima
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only