8,265 results
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Plasmid#227747PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG117
Plasmid#227746PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr, Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr, Val139Leu double mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
ExpressionYeastPromoterTDH3 promoterAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.002
Plasmid#228681PurposepCCW12 Promoter for yeast expression with Nterminal tagDepositorInsertpCCW12
ExpressionYeastMutationWTAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc
Plasmid#226718PurposePlasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.007
Plasmid#228686PurposepGAL1 Promoter for yeast expression with Nterminal tagDepositorInsertpGAL1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.006
Plasmid#228685PurposepTEF2 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.005
Plasmid#228684PurposepTEF1 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.004
Plasmid#228683PurposepHHF2 Promoter for yeast expression with Nterminal tagDepositorInsertpHHF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.003
Plasmid#228682PurposepPGK1 Promoter for yeast expression with Nterminal tagDepositorInsertpPGK1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.001
Plasmid#228680PurposepTDH3 Promoter for yeast expression with Nterminal tagDepositorInsertpTDH3
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13239
Plasmid#219912PurposeThe base plasmid of TUNEYALI for TF49DepositorInsertContains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
ExpressionYeastAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-TLCV2
Plasmid#196331Purposethe TLCV2 plasmid with Tol2 recombination sites for integrationDepositorInsertCas9-2A-eGFP
UseCRISPR; Fish expressionExpressionBacterial, Mammalian, and Y…PromoterTight TRE promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2-Strep (SB255)
Plasmid#227604PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)
Plasmid#227611PurposeInducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastorisDepositorTagsMBP, 10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-88 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
Plasmid#227610PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-400, 706-757 [TM and HB2 deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[∆2-83]-His (SB258)
Plasmid#227607PurposeInducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
Tags10xHisExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR58
Plasmid#202795Purposeintegrating bioluminescene reporter for Cas9 activity in S. stipitisDepositorInsertsP-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence
ExpressionYeastAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM198
Plasmid#227657PurposepRS305 His-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM200
Plasmid#227659PurposepRS305 3XFLAG-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
ExpressionYeastPromoterTEF1 S.c.Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TINGL-RR pDRF1
Plasmid#226435PurposeExpresses TINGL-RR (mTq2-based glucose sensor - O2A - ymScarletI) in yeastDepositorInsertTINGL-O2A-ymScarletI
ExpressionYeastPromoterPMA1Available SinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TINGL pDRF1
Plasmid#226436PurposeExpresses TINGL (mTq2-based glucose sensor) in yeastDepositorInsertTINGL
ExpressionYeastPromoterPMA1Available SinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TINGL-GR pDRF1
Plasmid#226437PurposeExpresses TINGL-RR (mTq2-based glucose sensor - O2A - ymNeongreen) in yeastDepositorInsertTINGL-O2A-ymNeongreen
ExpressionYeastPromoterPMA1Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BirA-HA3-HIS3MX6
Plasmid#227975PurposeTo insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBirA
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FRB-HA3
Plasmid#228008PurposeExpresses TurboID-FRB fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FRB-NLS-HA3
Plasmid#228009PurposeExpresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FKBP-HA3
Plasmid#228010PurposeExpresses TurboID-FKBP fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p- TID-FKBP-NLS-HA3
Plasmid#228011PurposeExpresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FRB-HA3
Plasmid#228012PurposeExpresses TurboID-FRB fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCHA1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FRB-NLS-HA3
Plasmid#228013PurposeExpresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCHA1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p- TID-FKBP-NLS-HA3
Plasmid#228015PurposeExpresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterCHA1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BioID2-HA3-HIS3MX6
Plasmid#227977PurposeTo insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBioID3
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-BASU-HA3-HIS3MX
Plasmid#227979PurposeTo insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX markerDepositorInsertBASU
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FRB-HA3
Plasmid#227996PurposeExpresses TurboID-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-KanMX6
Plasmid#228020PurposeFor TurboID-FRB integration; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-HIS3MX6
Plasmid#228021PurposeFor TurboID-FRB integration; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-HA3
Plasmid#227984PurposeExpresses BirA-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-HA3
Plasmid#227986PurposeExpresses BirA-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-NLS-HA3
Plasmid#227985PurposeExpresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-NLS-HA3
Plasmid#227987PurposeExpresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FRB-HA3
Plasmid#227988PurposeExpresses BioID2-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FRB-NLS-HA3
Plasmid#227989PurposeExpresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only