8,173 results
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Plasmid#216518PurposeExpression of LegA7DepositorInsertLeg A7
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 WTAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7Δank
Plasmid#216519PurposeExpression of LegA7 ΔAnkDepositorInsertLegA7 ΔAnk
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 331-454Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔAnk290
Plasmid#216520PurposeExpression of LegA7 ΔAnk290DepositorInsertLegA7 ΔAnk290
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-454Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk264
Plasmid#216521PurposeExpression of LegA7 ΔNAnk264DepositorInsertLegA7 ΔNAnk264
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 264-361Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk290
Plasmid#216522PurposeExpression of LegA7 ΔNAnk290DepositorInsertLegA7 ΔNAnk290
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-361Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-3
Plasmid#220440PurposeBase construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)DepositorTypeEmpty backboneExpressionYeastPromoterMV IE93 promoterAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-2
Plasmid#220438PurposeBase construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP80A_MS2
Plasmid#220624PurposetRNA reporter vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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RT-1
Plasmid#220434PurposeBase construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_ZF1_mNeonGreen
Plasmid#213834PurposeFacilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen_ZF2
Plasmid#213835PurposeFacilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_ZF2_mNeonGreen
Plasmid#213836PurposeFacilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen_ZF1
Plasmid#213837PurposeFacilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_mNeonGreen
Plasmid#213838PurposeFacilitates genomic integration of the control reporter without zinc finger binding sites.DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtet-1_mNeonGreen_Ptet-2_mCherry
Plasmid#213839PurposeExpression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.DepositorInsertmNeonGreen, mCherry
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen
Plasmid#213840PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-1DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen
Plasmid#213841PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-2DepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtet-2-VP16
Plasmid#213842PurposeUsed for expressing VP16 fused to ZF-2.DepositorInsertVP16
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtet-1-VP16
Plasmid#213843PurposeUsed for expressing VP16 fused to ZF-1.DepositorInsertVP16
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pStorage_Ptet-1_ZF1
Plasmid#213844PurposeCombiGEM storage plasmid for ZF-1 CR fusions.DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pStorage_Ptet-2_ZF2
Plasmid#213845PurposeCombiGEM storage plasmid for ZF-2 CR fusions.DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_TetR
Plasmid#213847PurposeFacilitates genomic integration of tetR expression cassette.DepositorInsertTetR
ExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12622
Plasmid#219929PurposePrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter ACT
ExpressionYeastAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13237
Plasmid#219910PurposeThe base plasmid of TUNEYALI for TF47DepositorInsertContains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47
ExpressionYeastAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12562
Plasmid#219923PurposePrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter GPD
ExpressionYeastAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p500-Erg6-V5-APEX2-URA
Plasmid#207070PurposeExpressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.DepositorInsertErg6
TagsV5-APEX2ExpressionYeastPromoterpERG6Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
ExpressionYeastPromoterpTEF1Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTPI1-Dsred-URA3
Plasmid#207017PurposeExpresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).DepositorInsertDsRed-Express 2
ExpressionYeastPromoterpTPI1Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13186
Plasmid#219924PurposePrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TEF
ExpressionYeastAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13204
Plasmid#219879PurposeThe base plasmid of TUNEYALI for TF14DepositorInsertContains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14
ExpressionYeastAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13200
Plasmid#219875PurposeThe base plasmid of TUNEYALI forTF10DepositorInsertContains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13242
Plasmid#219914PurposeThe base plasmid of TUNEYALI for TF52DepositorInsertContains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13245
Plasmid#219916PurposeThe base plasmid of TUNEYALI for TF55DepositorInsertContains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13193
Plasmid#219868PurposeThe base plasmid of TUNEYALI for TF03DepositorInsertContains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12561
Plasmid#219922PurposePrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter PGM
ExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12565
Plasmid#219925PurposePrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter FBA
ExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13223
Plasmid#219898PurposeThe base plasmid of TUNEYALI for TF33DepositorInsertContains gRNA targeting TF33 (YALI1_F14650g) and homologous arm matching TF33
ExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13197
Plasmid#219872PurposeThe base plasmid of TUNEYALI forTF07DepositorInsertContains gRNA targeting TF07 ( YALI1_D30097g) and homologous arm matching TF07
ExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13226
Plasmid#219901PurposeThe base plasmid of TUNEYALI for TF36DepositorInsertContains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36
ExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13194
Plasmid#219869PurposeThe base plasmid of TUNEYALI for TF04DepositorInsertContains gRNA targeting TF04 (YALI1_C02831g) and homologous arm matching TF04
ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13209
Plasmid#219884PurposeThe base plasmid of TUNEYALI for TF19DepositorInsertContains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13208
Plasmid#219883PurposeThe base plasmid of TUNEYALI for TF18DepositorInsertContains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18
ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13207
Plasmid#219882PurposeThe base plasmid of TUNEYALI for TF17DepositorInsertContains gRNA targeting TF17 (YALI1_B19962g) and homologous arm matching TF17
ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13244
Plasmid#219915PurposeThe base plasmid of TUNEYALI for TF54DepositorInsertContains gRNA targeting TF54 (YALI1_F26367g) and homologous arm matching TF54
ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13233
Plasmid#219906PurposeThe base plasmid of TUNEYALI for TF43DepositorInsertContains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41
ExpressionYeastAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13206
Plasmid#219881PurposeThe base plasmid of TUNEYALI for TF16DepositorInsertContains gRNA targeting TF16 (YALI1_B11759g) and homologous arm matching TF16
ExpressionYeastAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13196
Plasmid#219871PurposeThe base plasmid of TUNEYALI forTF06DepositorInsertContains gRNA targeting TF06 (YALI1_D34785g) and homologous arm matching TF06
ExpressionYeastAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only