8,058 results

1. pRMH120

   Plasmid

   #40593

   Purpose

   Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

   Depositor

   Chandra Tucker

   Article

   Hughes et al Methods. 2012 Aug 15.

   Inserts

   CRY2 (CRY2 Mustard Weed)
   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and TEF

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nuc-yHS1

   Plasmid

   #159166

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046451

   Plasmid

   #145567

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. p42Neo 1-F GW

   Plasmid

   #58550

   Purpose

   Multicopy with G418 resistance marker for single Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCHAP4

   Plasmid

   #206850

   Purpose

   A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.

   Depositor

   Bogumil Karas

   Article

   Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LD201

   Plasmid

   #69178

   Purpose

   Integration of 2GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg1-2EGFP
   

   Use

   Tags

   2xEGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-hphMX6-Purg1-3FLAG

   Plasmid

   #19356

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   urg1 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046348

   Plasmid

   #149108

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pJJB170

   Plasmid

   #158600

   Purpose

   Transcription factors ADR1c, PIP2c, OAF1c

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   ADR1c
   PIP2c
   OAF1c
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YIplac211-SYS1-msGFPx3

    Plasmid

    #177112

    Purpose

    Expresses Sys1-msGFP3 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    SYS1 (SYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKT0178

    Plasmid

    #8744

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJZC506

    Plasmid

    #62281

    Purpose

    COM-VP64 fusion

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    COM-VP64
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKT0176

    Plasmid

    #8743

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-yomKO2-Kan

    Plasmid

    #44958

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-3GFP-kanMX6

    Plasmid

    #105148

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, David Kovar, Thomas Pollard

    Article

    Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

    Insert

    3GFP-kanMX6
    

    Use

    Tags

    3GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Linked Free NES

    Plasmid

    #182485

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Linked Free PcPTS

    Plasmid

    #182486

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSNR52-sgTET

    Plasmid

    #46923

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgRNA targeting endogenous TRE elements of pTET07 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZJOM141

    Plasmid

    #133669

    Purpose

    Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXP841

    Plasmid

    #46067

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge