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pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179066

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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NM R(delta) 2-5 GFP

Plasmid

#1209

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-Cerulean-ccdB

Plasmid

#14418

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179060

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pKT0139

Plasmid

#8731

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSX6

Plasmid

#90468

Purpose

U6 wt in pSE358 (TRP1 CEN)

Depositor

Christine Guthrie

Article

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

June 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAV0758

Plasmid

#133511

Purpose

Expression of 3GFP tagged RasAct probe to monitor active Ras1

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0783

Plasmid

#133515

Purpose

Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPGP_scFv

Plasmid

#183732

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

GAP promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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FRP2000

Plasmid

#159309

Purpose

Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection

Depositor

Joerg Stelling

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAG414GAL-ccdB-HA

Plasmid

#14239

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0176

Plasmid

#8743

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Tags

Codon optimized linker

Expression

Yeast

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pAFZifPZ431

Plasmid

#185849

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED3>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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LD202

Plasmid

#69179

Purpose

Integration of 2GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg2-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046451

Plasmid

#145567

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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