8,060 results

1. pFA6a-kanMX6-P81nmt1-mYFP

   Plasmid

   #105144

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   kanMX6-P81nmt1-mYFP
   

   Use

   Tags

   81nmt1-mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   81nmt1

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYC48

   Plasmid

   #63905

   Purpose

   Recipient integrative vector to construct HA tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDA200

   Plasmid

   #74248

   Purpose

   pGPD1-dPSTRy(4,3)

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   pGPD1-dPSTRy(4,3)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH3FS-PYK1

   Plasmid

   #85781

   Purpose

   N-ICE plasmid pH3FS-PYK1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-PYK1p-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC54

   Plasmid

   #63909

   Purpose

   Recipient integrative vector to construct YFP tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-yomRuby-SpHis5

   Plasmid

   #44843

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomRuby
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT42_GalL_BE-PAPAPA

   Plasmid

   #145035

   Purpose

   Expresses BE-PAPAPA in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE-PAPAP
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHES820

   Plasmid

   #89190

   Purpose

   Expresses YFP under control of GAL1 promoter.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1-YFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK3FS-CYC1

   Plasmid

   #85779

   Purpose

   N-ICE plasmid pK3FS-CYC1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-CYC1p-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU-mRuby2

    Plasmid

    #176549

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH-mRuby2

    Plasmid

    #176547

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6677

    Plasmid

    #106155

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MtPT4

    Plasmid

    #117096

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    MtPT4
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p316CUP1-3SGFP.SG

    Plasmid

    #1207

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS34

    Plasmid

    #83796

    Purpose

    Useful for tagging yeast proteins with C-terminal mCherry tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pROS15

    Plasmid

    #107929

    Purpose

    nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJB1

    Plasmid

    #160429

    Purpose

    Yeast vector for expressing mCherry tagged genes under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIplac211-RPL13A-FKBPx2

    Plasmid

    #104473

    Purpose

    Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    RPL13Ap (RPL13A Budding Yeast)
    

    Use

    Tags

    FKBPx2-HA

    Expression

    Yeast

    Mutation

    includes the last 354 bp of RPL13A only

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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