8,418 results
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Plasmid#105255PurposePlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.DepositorAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only
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pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Leu2 drive
Plasmid#89578PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locusDepositorInsertLeu2 gene drive
ExpressionYeastAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-pol30(K164R)-3'
Plasmid#99548PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p416TEF-NYFP
Plasmid#99553PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorPublicationAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
ExpressionYeastAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIMVAd39T
Plasmid#98303PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.DepositorInsertpdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRExpressionYeastMutationChanged sequence to remove a MluI cut siteAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-PYK1
Plasmid#85781PurposeN-ICE plasmid pH3FS-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK013-3a-Invertase-αMFΔ
Plasmid#84972PurposeType 3a, Invertase followed by αMFΔ - secretion signalDepositorInsertInvertase followed by αMFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PATagRFP-KanMX6
Plasmid#87027PurposeTag S. pombe genes in C terminal with PATagRFPDepositorInsertPATagRFP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PP75
Plasmid#78945PurposeSupplemental Figure 3. Integration Site 2.DepositorInsertIntegration Site 2
ExpressionYeastAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
ExpressionYeastPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-FKBP(M)x4
Plasmid#85003PurposeExpresses EGFP-FKBP(M)x4 in yeastDepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only