8,418 results

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pCfB2795

Plasmid

#63650

Purpose

pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Publication

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNIA-CEN-Venus-FLAG-LINXa4-Bre1

Plasmid

#73618

Purpose

Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

Depositor

Brian Kuhlman

Publication

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

Venus

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2513

Plasmid

#67543

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB2223

Plasmid

#67544

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2226

Plasmid

#67533

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB2227

Plasmid

#67536

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2192

Plasmid

#67539

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pJK369

Plasmid

#73594

Purpose

Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

Depositor

T. J. Kappock

Insert

delta-12 fatty acid acetylenase

Tags

FLAG tag

Expression

Yeast

Promoter

GAL10

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJK372

Plasmid

#73597

Purpose

Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

Depositor

T. J. Kappock

Inserts

delta-12 fatty acid acetylenase
delta-15 fatty acid desaturase

Tags

FLAG tag and c-myc tag

Expression

Yeast

Promoter

GAL1 and GAL10

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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LD224

Plasmid

#69191

Purpose

Integration of 2GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg24-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD218

Plasmid

#69187

Purpose

Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg18-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LD214

Plasmid

#69185

Purpose

Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg14-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LD213

Plasmid

#69184

Purpose

Integration of 2GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg13-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LD205

Plasmid

#69180

Purpose

Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg5-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LD113

Plasmid

#69166

Purpose

Integration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg13-EGFP

Tags

EGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LD101

Plasmid

#69160

Purpose

Integration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg1-EGFP

Tags

EGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge