8,043 results

1. pCfb12566

   Plasmid

   #219926

   Purpose

   PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

   Depositor

   Irina Borodina

   Insert

   Promoter DGA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLS406

   Plasmid

   #231078

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfb13236

   Plasmid

   #219909

   Purpose

   The base plasmid of TUNEYALI for TF46

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLS405

   Plasmid

   #231077

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLS404

   Plasmid

   #231076

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLS408

   Plasmid

   #231079

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLS403

   Plasmid

   #231075

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLS400

   Plasmid

   #231080

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfb13199

    Plasmid

    #219874

    Purpose

    The base plasmid of TUNEYALI forTF09

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfb13218

    Plasmid

    #219893

    Purpose

    The base plasmid of TUNEYALI for TF28

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

    Plasmid

    #225222

    Purpose

    Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    α-Synuclein
    Aga2
    

    Use

    Tags

    GS linker, HA Tag, and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

    Plasmid

    #225228

    Purpose

    Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (25Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

    Plasmid

    #225229

    Purpose

    Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (46Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-SSD1-L1374

    Plasmid

    #226278

    Purpose

    Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SSD1 (SSD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVG329

    Plasmid

    #227771

    Purpose

    For genomic replacement of YOR1 gene in budding yeast

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVG134

    Plasmid

    #227770

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Glu84Gly mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVG178

    Plasmid

    #227761

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Glu84KLys mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVG144

    Plasmid

    #227760

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Val121Met mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVG150

    Plasmid

    #227759

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ser137Asn mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVG124

    Plasmid

    #227758

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Thr83Ser mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVG121

    Plasmid

    #227757

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Gly80Glu mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVG123

    Plasmid

    #227755

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ala157Val mutant

    Promoter

    PGK1pr

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pVG143

    Plasmid

    #227754

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leu87Ile

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVG147

    Plasmid

    #227753

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Gly94Cys mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVG120

    Plasmid

    #227752

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Asp89Tyr mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pVG137

    Plasmid

    #227751

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Val201Leu mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pVG136

    Plasmid

    #227750

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Pro165Ser mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVG135

    Plasmid

    #227749

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Lys99Glu mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVG119

    Plasmid

    #227748

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Val139Leu mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVG118

    Plasmid

    #227747

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Asp18Tyr mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVG117

    Plasmid

    #227746

    Purpose

    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr, Val139Leu) under a strong constitutive PGK1pr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al bioRxiv 2024.02.28.582517

    Insert

    EL222 codon-optimized for budding yeast
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Asp18Tyr, Val139Leu double mutant

    Promoter

    PGK1pr

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YIplac128-TEF2p-LDH-CYC1t

    Plasmid

    #216120

    Purpose

    Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Lactate dehydrogenase (LDH Bovine)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF2 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YIplac204-TDH3p-LDH-CYC1t

    Plasmid

    #216121

    Purpose

    Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Lactate dehydrogenase (LDH Bovine, Bos taurus)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. YIplac204-TDH3p-CDC19-CYC1t

    Plasmid

    #216122

    Purpose

    Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Pyruvate kinase 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMY.002

    Plasmid

    #228681

    Purpose

    pCCW12 Promoter for yeast expression with Nterminal tag

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    pCCW12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge