8,043 results

1. pOGS539_GLuc

   Plasmid

   #226718

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMY.007

   Plasmid

   #228686

   Purpose

   pGAL1 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pGAL1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMY.006

   Plasmid

   #228685

   Purpose

   pTEF2 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pTEF2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMY.005

   Plasmid

   #228684

   Purpose

   pTEF1 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pTEF1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMY.004

   Plasmid

   #228683

   Purpose

   pHHF2 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pHHF2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMY.003

   Plasmid

   #228682

   Purpose

   pPGK1 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pPGK1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMY.001

   Plasmid

   #228680

   Purpose

   pTDH3 Promoter for yeast expression with Nterminal tag

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   pTDH3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfb13239

   Plasmid

   #219912

   Purpose

   The base plasmid of TUNEYALI for TF49

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tol2-TLCV2

   Plasmid

   #196331

   Purpose

   the TLCV2 plasmid with Tol2 recombination sites for integration

   Depositor

   Itamar Harel

   Article

   Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR; Fish expression

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPICZ A-SLC-His+Strep-Opa1 (SB254)

    Plasmid

    #227603

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    Opa1
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-960 only

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid

    #227608

    Purpose

    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    aa 2-83 deleted

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid

    #227609

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-544, 651-757 only [TM deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPICZ A-SLC-His+MFN2-Strep (SB255)

    Plasmid

    #227604

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid

    #227611

    Purpose

    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    MBP, 10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-88 only

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid

    #227610

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-400, 706-757 [TM and HB2 deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPICZ A-SLC25A46[∆2-83]-His (SB258)

    Plasmid

    #227607

    Purpose

    Inducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    aa 2-83 deleted

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFa6-TID-FRB-NLS-3HA-KanMX6

    Plasmid

    #228022

    Purpose

    To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM198

    Plasmid

    #227657

    Purpose

    pRS305 His-HRV3C-Rpn5 WT

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Use

    Tags

    His-HRV3C-

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM200

    Plasmid

    #227659

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5 WT

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Use

    Tags

    3X FLAG-HRV3C-

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAM201

    Plasmid

    #227660

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Use

    Tags

    3x-FLAG-HRV3C-

    Expression

    Yeast

    Mutation

    (amino acids 121-131) VTENKIF mutated to AAAAAAA

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAM202

    Plasmid

    #227661

    Purpose

    pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SJZ6-HIS5

    Plasmid

    #228259

    Purpose

    Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    his5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TINGL-RR pDRF1

    Plasmid

    #226435

    Purpose

    Expresses TINGL-RR (mTq2-based glucose sensor - O2A - ymScarletI) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL-O2A-ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA1

    Available since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. TINGL pDRF1

    Plasmid

    #226436

    Purpose

    Expresses TINGL (mTq2-based glucose sensor) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA1

    Available since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. TINGL-GR pDRF1

    Plasmid

    #226437

    Purpose

    Expresses TINGL-RR (mTq2-based glucose sensor - O2A - ymNeongreen) in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    TINGL-O2A-ymNeongreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA1

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LHZ1494

    Plasmid

    #222104

    Purpose

    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

    Depositor

    Yongming Wang

    Insert

    sg1RNA, sg4RNA, sg7RNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-BirA-HA3-HIS3MX6

    Plasmid

    #227975

    Purpose

    To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS416-CUP1p-TID-FRB-HA3

    Plasmid

    #228008

    Purpose

    Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS416-CUP1p-TID-FRB-NLS-HA3

    Plasmid

    #228009

    Purpose

    Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS416-CUP1p-TID-FKBP-HA3

    Plasmid

    #228010

    Purpose

    Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS416-CUP1p- TID-FKBP-NLS-HA3

    Plasmid

    #228011

    Purpose

    Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS416-CHA1p-TID-FRB-HA3

    Plasmid

    #228012

    Purpose

    Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS416-CHA1p-TID-FRB-NLS-HA3

    Plasmid

    #228013

    Purpose

    Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS416-CHA1p- TID-FKBP-NLS-HA3

    Plasmid

    #228015

    Purpose

    Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-BioID2-HA3-HIS3MX6

    Plasmid

    #227977

    Purpose

    To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BioID3
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFa6-BASU-HA3-HIS3MX

    Plasmid

    #227979

    Purpose

    To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BASU
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CPY-ymsfGFP

    Plasmid

    #209538

    Purpose

    Expresses CYP tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CPY*-ymsfGFP

    Plasmid

    #209539

    Purpose

    Expresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY*
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRS416-ADH1p-TID-FRB-HA3

    Plasmid

    #227996

    Purpose

    Expresses TurboID-FRB fusion from ADH1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge