8,265 results

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pCfB2803

Plasmid

#63646

Purpose

pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHES820

Plasmid

#89190

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pYC48

Plasmid

#63905

Purpose

Recipient integrative vector to construct HA tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

HA

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC61

Plasmid

#63925

Purpose

Recipient replicative vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pYC55

Plasmid

#63922

Purpose

Recipient replicative vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pYC42

Plasmid

#63920

Purpose

Recipient replicative vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pL-Sapphire

Plasmid

#176568

Purpose

Expression of Sapphire for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Insert

yETSapphire

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pICE LYS2

Plasmid

#122879

Purpose

LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Tags

6xHIS

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pROS15

Plasmid

#107929

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0714

Plasmid

#133479

Purpose

Expression in fission yeast from promoter pact1, integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-ccdB-mRuby3

Plasmid

#166139

Purpose

Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

mRuby3

Expression

Yeast

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD8A-RGR-r10

Plasmid

#74370

Purpose

This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

RGR-r10

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

ADH1

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pICE TRP1

Plasmid

#122881

Purpose

TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDH3

Plasmid

#83789

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Mutation

F64L, Y66W, N146I, M153T, V163A, N164H mutations …

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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