8,043 results

1. pPICZ A-MFN2-Strep (SB253)

   Plasmid

   #227602

   Purpose

   Inducible expression of Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   Twin-StrepTag, 10xHis

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPICZ A-Strep-Opa1(MGD) (SB252)

   Plasmid

   #227601

   Purpose

   Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-580 (MGD) and aa 938-960

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfb13247

   Plasmid

   #219918

   Purpose

   The base plasmid of TUNEYALI for TF57

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfb13228

   Plasmid

   #219903

   Purpose

   The base plasmid of TUNEYALI for TF38

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfb13210

   Plasmid

   #219885

   Purpose

   The base plasmid of TUNEYALI for TF20

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFa6-TID-FKBP-3HA-KanMX6

   Plasmid

   #228024

   Purpose

   To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFa6-TID-FKBP-NLS-3HA-KanMX6

   Plasmid

   #228026

   Purpose

   To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-BASU-HA3-KanMX6

   Plasmid

   #227978

   Purpose

   To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   BASU
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-BioID2-HA3-KanMX6

   Plasmid

   #227976

   Purpose

   To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   BioID2
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-BirA-HA3-KanMX6

    Plasmid

    #227974

    Purpose

    To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid

    #227084

    Purpose

    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

    Depositor

    Chris Hill

    Insert

    spt6-tsh2 (SPT6 Budding Yeast)
    

    Use

    Tags

    STREPii-FLAG and SV40 NLS 1

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CUP1-∆ssCPY*-mScarletI

    Plasmid

    #221157

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CUP1-∆ssCPY*-mNeonGreen

    Plasmid

    #221156

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid

    #221160

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CUP1-∆ssCPY*-mGFP

    Plasmid

    #212197

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Chris MacDonald, Mark Leake

    Insert

    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    

    Use

    Tags

    mEGFP (monomeric version EGFP, A206K mutation)

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    CUP1

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPOM034_P7_3"Lys3

    Plasmid

    #216459

    Purpose

    3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    3''lys3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSII41K

    Plasmid

    #194522

    Purpose

    Low copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSII41N

    Plasmid

    #194524

    Purpose

    Low copy vector backbone with nourseothricin selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSII42N

    Plasmid

    #194525

    Purpose

    High copy vector backbone with nourseothricin selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSII41H

    Plasmid

    #194526

    Purpose

    Low copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSII42H

    Plasmid

    #194527

    Purpose

    High copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSII41K-TDH3pr

    Plasmid

    #194528

    Purpose

    Low copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSII42K-TDH3pr

    Plasmid

    #194529

    Purpose

    High copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSII41N-TDH3pr

    Plasmid

    #194530

    Purpose

    Low copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSII42N-TDH3pr

    Plasmid

    #194531

    Purpose

    High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSII41H-TDH3pr

    Plasmid

    #194532

    Purpose

    Low copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSII41K-TDH3pr-mNeon

    Plasmid

    #194534

    Purpose

    Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSII42K-TDH3pr-mNeon

    Plasmid

    #194535

    Purpose

    High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSII41N-TDH3pr-mNeon

    Plasmid

    #194536

    Purpose

    Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    natAC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSII42N-TDH3pr-mNeon

    Plasmid

    #194537

    Purpose

    High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    natAC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRSII41H-TDH3pr-mNeon

    Plasmid

    #194538

    Purpose

    Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS315-SEC22-NCYC110

    Plasmid

    #226273

    Purpose

    Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS316-SEC18-L1374

    Plasmid

    #226275

    Purpose

    Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC18 (SEC18 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS316-SEC18-S288C

    Plasmid

    #226274

    Purpose

    Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC18 (SEC18 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS315-SEC22-L1374

    Plasmid

    #226271

    Purpose

    Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS315-SEC22-UWOPS872421

    Plasmid

    #226272

    Purpose

    Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRS316-SEC18-NCYC110

    Plasmid

    #226277

    Purpose

    Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC18 (SEC18 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS316-SEC18-UWOPS872421

    Plasmid

    #226276

    Purpose

    Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC18 (SEC18 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS315-SEC22-S288C

    Plasmid

    #226270

    Purpose

    Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRS313-SCT1-S288C

    Plasmid

    #226266

    Purpose

    Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SCT1 (SCT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge