8,086 results

1. pPTK018-3b-RFP_Sec

   Plasmid

   #84977

   Purpose

   Type 3b, Red fluorescent protein

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   BsaI site removed (1677 c to g and 1794 a to g)

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNA0304

   Plasmid

   #165612

   Purpose

   Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   NotI site replaced the CAN1 spacer in parent vect…

   Promoter

   SNR52

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPTK001-2-pAOX1

   Plasmid

   #84960

   Purpose

   Type 2, pAOX1, Alcohol oxidase 1 promoter

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Alcohol oxidase 1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-link-yoClover-SpHis5

   Plasmid

   #44857

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoClover
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. M4753 KanMX::ADE2 Disruptor Converter

   Plasmid

   #51683

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to ADE2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   ADE2 (ADE2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pROS13

   Plasmid

   #107927

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-hphMX6-P81nmt1-3FLAG

   Plasmid

   #19350

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJZC595

   Plasmid

   #62322

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Has nuclease-inactivating mutations in the RuvC1 …

   Promoter

   Tdh3 and pAdh

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXP742

   Plasmid

   #46060

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH2

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICXC3H

    Plasmid

    #49022

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. THI VP64 pGAP T-

    Plasmid

    #161487

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC-X2

    Plasmid

    #176152

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-X3

    Plasmid

    #176153

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC-XI1

    Plasmid

    #176155

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC-XI5

    Plasmid

    #176158

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC-XII2

    Plasmid

    #176160

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV0523

    Plasmid

    #133495

    Purpose

    Expression of nucleo-cytoplasmic sfGFP in M-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(mam1*)-sfGFP-terminator(ScADH1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHES834

    Plasmid

    #87947

    Purpose

    Expresses mKate2 under control of GAL1 promoter.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pGAL1-mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge