8,043 results
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Plasmid#219854Purposeexpression of Cyterm-ymiRFP680 in budding yeastDepositorInsertCyterm-ymiRFP680
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1 ymScarletI3_T2A_mTurquoise2
Plasmid#219829Purposeequimolar expression of ymScarletI3 and mTurquoise2 in budding yeastDepositorInsertymScarletI3_T2A_mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1 StayGold-E138D-T2A-mCherry
Plasmid#219833Purposeequimolar expression of Staygold-E138D and mCherry in budding yeastDepositorInsertStayGold-E138D-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ytd8oxStayGold-T2A-mCherry
Plasmid#219838Purposeequimolar expression of ytd8oxStayGold and mCherry in budding yeastDepositorInsertytd8oxStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 yStayGold-T2A-mCherry
Plasmid#219839Purposeequimolar expression of yStayGold and mCherry in budding yeastDepositorInsertyStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LHZ1493
Plasmid#222102PurposeCloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETURALEU
Plasmid#223207PurposePlasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markersDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKDC.100
Plasmid#222224PurposeAcceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL194
Plasmid#222225PurposeExpresses site 1/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 1/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL195
Plasmid#222226PurposeExpresses site 2/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 2/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL368
Plasmid#222227PurposeExpresses site 3/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 3/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-ATXN1-Q85
Plasmid#181712PurposeGalactose inducible expression of ATXN1-Q85DepositorInsertATXN1-Q85 (ATXN1 Human)
UseTagsExpressionYeastMutationextended repeat of 85 Gln residues replacing nati…PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7181
Plasmid#219766PurposeVector for expression HmS in yeast (P.pastoris)DepositorInsertHmS
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7182
Plasmid#219767PurposeVector for expression PpASCL in yeast (P.pastoris)DepositorInsertPpASCL
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7188
Plasmid#219768PurposeVector for expression Pv4CL1 in yeast (P.pastoris)DepositorInsertPv4CL1
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13220
Plasmid#219895PurposeThe base plasmid of TUNEYALI for TF30DepositorInsertContains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mKelly-T2A-mtq2
Plasmid#205506PurposeExpression of mKelly-T2A-mtq2 in yeastDepositorInsertmKelly-T2A-mtq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-APP-CTF
Plasmid#181732PurposeGalactose inducible expression of APP-CTFDepositorInsertAPP-CTF (APP Human)
UseTagsExpressionYeastMutationC-terminal fragment (aa 671-770)PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR95(URA3)
Plasmid#203917PurposeBLINCAR plasmid for gene deletion, URA3 targetDepositorInsertsURA3 upstream homology
URA3 downstream homology
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR91
Plasmid#203915Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locusDepositorInsertCRF1 plus flanking (CRF1 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR90
Plasmid#203914Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locusDepositorInsertGSC2 plus flanking (GSC2 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR89
Plasmid#203423Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locusDepositorInsertEGC3 plus flanking (EGC3 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR88
Plasmid#203422Purposeempty BLINCAR plasmid for payload delivery, no payload, no genomic targetDepositorInsertsCAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR63(YUM1)
Plasmid#203336Purposeepisomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR63
Plasmid#203331Purposeepisomal Cas9 and sgRNA expression vector without sgRNA for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR76
Plasmid#202776Purposeepisomal fluorescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR23
Plasmid#202770Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR10
Plasmid#202710Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR9
Plasmid#202645Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7
Plasmid#216518PurposeExpression of LegA7DepositorInsertLeg A7
UseTagsHIS-tagExpressionBacterial and YeastMutationLegA7 WTPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7Δank
Plasmid#216519PurposeExpression of LegA7 ΔAnkDepositorInsertLegA7 ΔAnk
UseTagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 331-454PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔAnk290
Plasmid#216520PurposeExpression of LegA7 ΔAnk290DepositorInsertLegA7 ΔAnk290
UseTagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-454PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk264
Plasmid#216521PurposeExpression of LegA7 ΔNAnk264DepositorInsertLegA7 ΔNAnk264
UseTagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 264-361PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk290
Plasmid#216522PurposeExpression of LegA7 ΔNAnk290DepositorInsertLegA7 ΔNAnk290
UseTagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-361PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-3
Plasmid#220440PurposeBase construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterMV IE93 promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTEF1_MS2
Plasmid#220625PurposeSource for TEF1 MS2 coat fusion protein fusionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-2
Plasmid#220438PurposeBase construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-5
Plasmid#220442PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)DepositorInsertPUF3 (puf-3 Nematode)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorInsertPUF3 (PUF3 Budding Yeast)
UseTags3xHAExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP80A_MS2
Plasmid#220624PurposetRNA reporter vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only