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8,173 results

Showing: 6301 - 6350 of 8173 results

  5. LifeAct-mTq2 pDRF1-GW

    Plasmid
    #168084
    Purpose
    Expresses Lifeact-mTq2 to visualize actin in yeast
    Depositor
    Bas Teusink
    Insert
    LifeAct-mTq2
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168084-plasmid-map-sequence-id-332098

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  6. LifeAct-mScarlet pDRF1-GW

    Plasmid
    #168086
    Purpose
    Expresses Lifeact-mScarlet to visualize actin in yeast
    Depositor
    Bas Teusink
    Insert
    LifeAct-mScarlet
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168086-plasmid-map-sequence-id-332145

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  7. yomCherry pDRF1-GW

    Plasmid
    #168087
    Purpose
    Expresses yomCherry
    Depositor
    Bas Teusink
    Insert
    yomCherry
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168087-plasmid-map-sequence-id-332099

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  8. AausFP1 pDRF1-GW

    Plasmid
    #168088
    Purpose
    Expresses AausFP1
    Depositor
    Bas Teusink
    Insert
    AausFP1
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168088-plasmid-map-sequence-id-331869

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  9. yotagRFPT pDRF1-GW

    Plasmid
    #168089
    Purpose
    Expresses yotagRFPT
    Depositor
    Bas Teusink
    Insert
    yotagRFPT
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168089-plasmid-map-sequence-id-332103

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  10. Cyterm-yotagRFPT pDRF1-GW

    Plasmid
    #168090
    Purpose
    Expresses Cyterm-yotagRFPT to check for monomericity.
    Depositor
    Bas Teusink
    Insert
    yotagRFPT
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168090-plasmid-map-sequence-id-332003

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  11. Cyterm-mGreenLantern pDRF1-GW

    Plasmid
    #168091
    Purpose
    Expresses Cyterm-mGreenLantern to check for monomericity.
    Depositor
    Bas Teusink
    Insert
    mGreenLantern
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168091-plasmid-map-sequence-id-332096

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  13. mGreenLantern pDRF1-GW

    Plasmid
    #168093
    Purpose
    Expresses mGreenLantern to check for monomericity.
    Depositor
    Bas Teusink
    Insert
    mGreenLantern
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168093-plasmid-map-sequence-id-331866

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  17. ymTq2-AausFP1 pDRF1-GW

    Plasmid
    #168064
    Purpose
    FRET fusion between ymTq2 and AausFP1 for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymTq2-AausFP1
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168064-plasmid-map-sequence-id-332005

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  18. ymTq2-yotagRFPT pDRF1-GW

    Plasmid
    #168065
    Purpose
    FRET fusion between ymTq2 and yotagRFPT for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymTq2-yotagRFPT
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168065-plasmid-map-sequence-id-332107

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  19. ymTq2-yomCherry pDRF1-GW

    Plasmid
    #168066
    Purpose
    FRET fusion between ymTq2 and yomCherry for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymTq2-yomCherry
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168066-plasmid-map-sequence-id-332097

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  20. ymTq2-tdTomato pDRF1-GW

    Plasmid
    #168068
    Purpose
    FRET fusion between ymTq2 and tdTomato for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymTq2-tdTomato
    Expression
    Yeast
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168068-plasmid-map-sequence-id-332369

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  21. TY5_hisAI_2micron

    Plasmid
    #138622
    Purpose
    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment
    Depositor
    Rob Mitra
    Insert
    TY5
    Expression
    Yeast
    Promoter
    Gal1_10
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138622-plasmid-map-sequence-id-334952

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  23. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  24. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  26. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid
    #169358
    Purpose
    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Expression
    Yeast
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169358-plasmid-map-sequence-id-333930

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  28. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid
    #169359
    Purpose
    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    3XHA
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169359-plasmid-map-sequence-id-333203

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  29. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

    Plasmid
    #169361
    Purpose
    C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169361-plasmid-map-sequence-id-333201

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  30. Padh1-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169349
    Purpose
    Expression in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169349-plasmid-map-sequence-id-332962

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  31. Padh81-MCS-TADHdh1-Sp.arg3+/pUC19*

    Plasmid
    #169351
    Purpose
    Expression in S. pombe from adh81 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169351-plasmid-map-sequence-id-332961

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  32. Padh15-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169350
    Purpose
    Expression in S. pombe from adh15 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169350-plasmid-map-sequence-id-332956

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  33. pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6

    Plasmid
    #169360
    Purpose
    C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    5XMyc
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169360-plasmid-map-sequence-id-332955

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  34. pMEL15_MATz

    Plasmid
    #166099
    Purpose
    Expression of gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166099-plasmid-map-sequence-id-328795

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  35. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  36. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  37. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

    Enlarge

  45. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

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  46. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

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  47. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  48. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

    Enlarge

  49. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

    Enlarge

  50. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

    Enlarge

Showing: 6301 - 6350 of 8173 results