8,432 results
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Plasmid#241276PurposeYeast two hybrid vector expressing AD-HAB1DepositorAvailable sinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only
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pFS553
Plasmid#242853PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ymBaojin-T2A-mCherry
Plasmid#219857Purposeequimolar expression of ymBaojin and mCherry in budding yeastDepositorInsertymBaojin-T2A-mCherry
ExpressionYeastAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS416
Plasmid#239127PurposeMinimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorTypeEmpty backboneUseCentromericExpressionBacterial and YeastAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30-LaccID
Plasmid#234652PurposeExpresses LaccID evolved variant in yeastDepositorInsertLaccID
TagsHRV 3C protease site-GSG linker-8xHis tagExpressionYeastMutationV11A, Q45R, N74S, R121S, I126V, D255G, L300VPromoterGal1pAvailable sinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13BE5
Plasmid#223836PurposeExpressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>ApacH>StayGold2
ExpressionYeastAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc [Pol31 + Pol32]-pRS403/GAL
Plasmid#239199PurposeOvererxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)DepositorAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Sc Pol3-FLAG(Ec)-pRSF405/GAL
Plasmid#239198PurposeOverexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)DepositorInsertPol3 (POL3 Budding Yeast)
Tags3X FLAGExpressionYeastMutationE. coli codon bias (synthetic gene), but same ami…Available sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAL003
Plasmid#233864PurposeMoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2DepositorInsertpRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2
ExpressionYeastAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
P415 ymBaojin
Plasmid#224086PurposeExpressing ymBaojin in yeastDepositorInsertymBaoJin
ExpressionYeastAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT899
Plasmid#228288PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-HIS3
Plasmid#232093PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Hyg
Plasmid#232091PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only