8,086 results

1. KBB285

   Plasmid

   #185076

   Purpose

   RFA3-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA3
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. KBB284

   Plasmid

   #185075

   Purpose

   RFA2-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA2
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LD220

   Plasmid

   #69188

   Purpose

   Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg20-2EGFP
   

   Use

   Tags

   2xEGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LHP336 - HA-Ub-K48R

   Plasmid

   #32164

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   K48R

   Promoter

   CUP1

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pTH644-CENBEVY

   Plasmid

   #29695

   Depositor

   Tobias von der Haar

   Article

   Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

   Insert

   bidirectional promoter cassette from pBEVY-U
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTCW002

   Plasmid

   #83522

   Purpose

   pheromone mediated destablised-GFP expression in yeast

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pFUS1-yEGFPCLN2PEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS314-hUD

   Plasmid

   #11005

   Depositor

   Francisco Malagon

   Insert

   hUD recombination system
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Direct repeat recombination system. Recombination…

   Promoter

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ273: pRS426-His-MBP-GtFz1_PSP1

   Plasmid

   #205258

   Purpose

   S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   GtFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMJ127: pRS426-His-MBP-NlovFz2_PSP1

   Plasmid

   #205257

   Purpose

   S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   NlovFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP1F5

    Plasmid

    #165064

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNC824

    Plasmid

    #20384

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    UAS cassette (FUS1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiY (see schematic)

    Promoter

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCoofy42

    Plasmid

    #55190

    Purpose

    Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMS22H

    Plasmid

    #133904

    Purpose

    Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK148-Pnmt41-GBP-mCherry(N)-leu1+

    Plasmid

    #89070

    Purpose

    genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046381

    Plasmid

    #149010

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCfB3036(XI-1 MarkerFree)

    Plasmid

    #73274

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416GPD-ccdB-EYFP

    Plasmid

    #14220

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPS293

    Plasmid

    #8851

    Depositor

    Pamela Silver

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGBW-m4046692

    Plasmid

    #149195

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

    Plasmid

    #89071

    Purpose

    genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge