8,133 results

1. pXRL2

   Plasmid

   #63148

   Purpose

   Leu2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Bacterial and Yeast

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABF2-2EGFP-ter-URA3

   Plasmid

   #184768

   Purpose

   Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   ABF2 (ABF2 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046692

   Plasmid

   #149195

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pNC824

   Plasmid

   #20384

   Depositor

   Beverly Errede

   Article

   Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

   Insert

   UAS cassette (FUS1)
   

   Expression

   Yeast

   Mutation

   X=UbiY (see schematic)

   Available Since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTCW002

   Plasmid

   #83522

   Purpose

   pheromone mediated destablised-GFP expression in yeast

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pFUS1-yEGFPCLN2PEST
   

   Expression

   Yeast

   Promoter

   pFUS1

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS22H

   Plasmid

   #133904

   Purpose

   Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PhyB-mCherry-SNF7

   Plasmid

   #51574

   Purpose

   Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   SNF7 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPT-PCR C2

   Plasmid

   #141247

   Purpose

   PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Algal Research, 2020. 50, 102014

   Insert

   Mitochondrial Genome of Phaeodactylum tricornutum
   

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Mutation

   Repeat region removed, additional minor mutations…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSK260

   Plasmid

   #129252

   Purpose

   dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Sup35 NM yTRAP sensor reporting on mKate2
   

   Expression

   Yeast

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPS808

    Plasmid

    #8856

    Depositor

    Pamela Silver

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pMZ289

    Plasmid

    #59898

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. KBB285

    Plasmid

    #185076

    Purpose

    RFA3-GFP fusion

    Depositor

    Ken Belanger

    Article

    Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

    Insert

    RFA3
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. KBB284

    Plasmid

    #185075

    Purpose

    RFA2-GFP fusion

    Depositor

    Ken Belanger

    Article

    Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

    Insert

    RFA2
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICE ADE2-HphMx

    Plasmid

    #122895

    Purpose

    ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS2

    Plasmid

    #12389

    Depositor

    Nick Rhind

    Article

    Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

    Insert

    green fluorescent protein
    

    Expression

    Yeast

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL 103Q+ProCFPp303

    Plasmid

    #15575

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ273: pRS426-His-MBP-GtFz1_PSP1

    Plasmid

    #205258

    Purpose

    S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJ127: pRS426-His-MBP-NlovFz2_PSP1

    Plasmid

    #205257

    Purpose

    S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFP1F5

    Plasmid

    #165064

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
    

    Expression

    Yeast

    Mutation

    WT

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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