8,060 results

1. pJZC625

   Plasmid

   #62321

   Purpose

   sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA +1x MS2, Pol II promoter with ribozyme cleavage
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pAdh

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-bleMX6-Purg1-3FLAG

   Plasmid

   #19357

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   urg1 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2989

   Plasmid

   #63636

   Purpose

   pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046332

   Plasmid

   #145413

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFA6a-hphMX6-P41nmt1-3FLAG

   Plasmid

   #19349

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXP822

   Plasmid

   #46064

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. EZ-L767

   Plasmid

   #131773

   Purpose

   PADH1_sFUS_Fusion Red_PixD_TACT1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_sFUS_Fusion Red_PixD_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p405GALL

   Plasmid

   #15964

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405GALL (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR208IIM1

   Plasmid

   #45352

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

   Insert

   UBR1 mutant
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   P406S, DE317NK

   Promoter

   ADH

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Use

    Tags

    yEGFP reporter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pITGFP3

    Plasmid

    #65452

    Purpose

    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Use

    Tags

    yEGFP-CLN2PEST reporter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid

    #169355

    Purpose

    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    3XHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW8-aid-Turg1:kanMX6

    Plasmid

    #169354

    Purpose

    C-terminal aid degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWS627

    Plasmid

    #123064

    Purpose

    G___ bicistronic protein

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Ste4-2A-Ste18 [3-4a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. RPL25NLS-GFP (101)

    Plasmid

    #24038

    Depositor

    Michael Rout

    Insert

    RPL25 (RPL25 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Only bp 1-135 (aa 1-45) of RPL25

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG414GAL-ccdB-DsRed

    Plasmid

    #14359

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. YIplac211-iGFP-YPT31

    Plasmid

    #105263

    Purpose

    Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Ypt31 (YPT31 )
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSJD001

    Plasmid

    #158589

    Purpose

    Pex22(1-36)-mRuby2 peroxisome marker

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    Pex22(1-36)-mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLK89 - Edit-directing plasmid cloning insert

    Plasmid

    #98846

    Purpose

    Contains the insert used for step 2 of edit-directed plasmid cloning

    Depositor

    Leonid Kruglyak

    Insert

    KanR
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Changed sequence to remove a MluI cut site

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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