8,086 results

1. pDS191

   Plasmid

   #34978

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITI1

   Plasmid

   #166094

   Purpose

   Gal inducible expression of dCas9-FokI

   Depositor

   Rebecca Burgess

   Insert

   dCas9-FokI
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A, H840A

   Promoter

   Gal-inducible

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB330

   Plasmid

   #60147

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   NLuc
   

   Use

   Tags

   Nuclear localization signal (NLS)

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AtCOPT1 upGFP

   Plasmid

   #117899

   Purpose

   protein expression

   Depositor

   Geoffrey Chang

   Insert

   AtCOPT1 upGFP (COPT1 Mustard Weed)
   

   Use

   Tags

   eGFP

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVG48

   Plasmid

   #164706

   Purpose

   rtTA under the control of PGK1 promoter

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   PGK1pr - rtTA - CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG426GPD-ccdB-EYFP

   Plasmid

   #14228

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. p416TEF-SEC16

   Plasmid

   #102676

   Purpose

   The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

   Depositor

   Jens Nielsen

   Article

   Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

   Insert

   SEC16 (SEC16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p42Nat 3-F GW

   Plasmid

   #58557

   Purpose

   Multicopy with clonNat resistance marker for triple Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINER2R

   Plasmid

   #185882

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNB813

    Plasmid

    #60161

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pI3-MTU-DO-HPH

    Plasmid

    #160207

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pI3-MTU-DO-LEU

    Plasmid

    #160176

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pI4-MTU-DO-LEU

    Plasmid

    #160029

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pI3-MTU-DO-HIS

    Plasmid

    #160021

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pI3-MTU-DO-URA

    Plasmid

    #160020

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. p406MET25

    Plasmid

    #17421

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG303GAL-ccdB-EYFP

    Plasmid

    #14205

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge