8,047 results

1. ystagRFP-T2A-mTq2 pdrf1-GW

   Plasmid

   #168072

   Purpose

   Produces equimolar expression of ystagRFP and mCherry for use in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   ystagRFP-T2A-mTq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mAvicFP1-T2A-mCherry pdrf1-GW

   Plasmid

   #168073

   Purpose

   Produces equimolar expression of mAvicFP1 and mCherry for use in yeast

   Depositor

   Bas Teusink

   Insert

   mAvicFP1-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ymTq2-mAvicFP1 pDRF1-GW

   Plasmid

   #168075

   Purpose

   FRET fusion between ymTq2 and mAvicFP1 for use in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   ymTq2-mAvicFP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cyterm-mTq2 pDRF1-GW

   Plasmid

   #168078

   Purpose

   Expresses Cyterm-mTq2 to check for monomericity.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   cyterm-mTq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cyterm-ymVenus pDRF1-GW

   Plasmid

   #168079

   Purpose

   Expresses Cyterm-mVenus to check for monomericity.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   cyterm-mVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. cyterm-yeVenus pDRF1-GW

   Plasmid

   #168080

   Purpose

   Expresses Cyterm-yeVenus to check for monomericity.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   cyterm-yeVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. cyterm-tagRFPT pDRF1-GW

   Plasmid

   #168081

   Purpose

   Expresses Cyterm-tagRFPT to check for monomericity.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   cyterm-tagRFPT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LifeAct-mTq2 pDRF1-GW

   Plasmid

   #168084

   Purpose

   Expresses Lifeact-mTq2 to visualize actin in yeast

   Depositor

   Bas Teusink

   Insert

   LifeAct-mTq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LifeAct-mScarlet pDRF1-GW

   Plasmid

   #168086

   Purpose

   Expresses Lifeact-mScarlet to visualize actin in yeast

   Depositor

   Bas Teusink

   Insert

   LifeAct-mScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. yomCherry pDRF1-GW

    Plasmid

    #168087

    Purpose

    Expresses yomCherry

    Depositor

    Bas Teusink

    Insert

    yomCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AausFP1 pDRF1-GW

    Plasmid

    #168088

    Purpose

    Expresses AausFP1

    Depositor

    Bas Teusink

    Insert

    AausFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. yotagRFPT pDRF1-GW

    Plasmid

    #168089

    Purpose

    Expresses yotagRFPT

    Depositor

    Bas Teusink

    Insert

    yotagRFPT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cyterm-yotagRFPT pDRF1-GW

    Plasmid

    #168090

    Purpose

    Expresses Cyterm-yotagRFPT to check for monomericity.

    Depositor

    Bas Teusink

    Insert

    yotagRFPT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cyterm-mGreenLantern pDRF1-GW

    Plasmid

    #168091

    Purpose

    Expresses Cyterm-mGreenLantern to check for monomericity.

    Depositor

    Bas Teusink

    Insert

    mGreenLantern
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cyterm-mAvicFP1 pDRF1-GW

    Plasmid

    #168092

    Purpose

    Expresses Cyterm-mAvicFP1 to check for monomericity.

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mAvicFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mGreenLantern pDRF1-GW

    Plasmid

    #168093

    Purpose

    Expresses mGreenLantern to check for monomericity.

    Depositor

    Bas Teusink

    Insert

    mGreenLantern
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mGreenLantern-T2A-mCherry pDRF1-GW

    Plasmid

    #168060

    Purpose

    Produces equimolar expression of mGreenLantern and mCherry for use in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mGreenLantern-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cyterm-ystagRFP pDRF1-GW

    Plasmid

    #168061

    Purpose

    Expresses Cyterm-ystagRFP to check for monomericity.

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    Cyterm-ystagRFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cyterm-AausFP1 pDRF1-GW

    Plasmid

    #168062

    Purpose

    Expresses Cyterm-AausFP1 to check for monomericity.

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    Cyterm-AausFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ymTq2-AausFP1 pDRF1-GW

    Plasmid

    #168064

    Purpose

    FRET fusion between ymTq2 and AausFP1 for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-AausFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ymTq2-yotagRFPT pDRF1-GW

    Plasmid

    #168065

    Purpose

    FRET fusion between ymTq2 and yotagRFPT for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-yotagRFPT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ymTq2-yomCherry pDRF1-GW

    Plasmid

    #168066

    Purpose

    FRET fusion between ymTq2 and yomCherry for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-yomCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ymTq2-tdTomato pDRF1-GW

    Plasmid

    #168068

    Purpose

    FRET fusion between ymTq2 and tdTomato for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TY5_hisAI_2micron

    Plasmid

    #138622

    Purpose

    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment

    Depositor

    Rob Mitra

    Insert

    TY5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1_10

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETCON-Aga2p-TAPBPR-myc

    Plasmid

    #153486

    Purpose

    Yeast expression of Aga2p-TAPBPR-myc

    Depositor

    Erik Procko

    Article

    McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Aga2P and c-myc epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCSN071

    Plasmid

    #166729

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSN072

    Plasmid

    #166730

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dcp2 ΔH1 WD

    Plasmid

    #163580

    Purpose

    express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W50A, D54A, L255A

    Promoter

    DCP2 promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid

    #169358

    Purpose

    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNIA-CEN-FLAG-LANS1-Set2

    Plasmid

    #122002

    Purpose

    FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    histone methyltransferase SET2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid

    #169359

    Purpose

    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    3XHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

    Plasmid

    #169361

    Purpose

    C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    1XV5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Padh1-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169349

    Purpose

    Expression in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Padh81-MCS-TADHdh1-Sp.arg3+/pUC19*

    Plasmid

    #169351

    Purpose

    Expression in S. pombe from adh81 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Padh15-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169350

    Purpose

    Expression in S. pombe from adh15 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6

    Plasmid

    #169360

    Purpose

    C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    5XMyc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMEL15_MATz

    Plasmid

    #166099

    Purpose

    Expression of gRNA targeting the MAT locus

    Depositor

    Rebecca Burgess

    Insert

    MAT Z1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNSU299_MET16

    Plasmid

    #166091

    Purpose

    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    MET16 (MET16 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGG431

    Plasmid

    #165615

    Purpose

    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGG442

    Plasmid

    #165616

    Purpose

    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    

    Use

    CRISPR

    Tags

    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge