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p41Neo MagicFluor
Plasmid#58564Purposep41Neo carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046620
Plasmid#153894PurposeYeast expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
104b-U1(WT)
Plasmid#1153DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046581
Plasmid#149064PurposeYeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pK218TK620T
Plasmid#1192DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationchanged lysine 218 to threonine and lysine 620 to…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMZ377
Plasmid#74214PurposeCombined sgRNA/Cas9 vector with LEU2 markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 G294A
Plasmid#27459DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ACT1_intron
Plasmid#127602Purposelow-copy URA-selectable plasmid, ACT1 intron inserted into URA3DepositorInsertACT1 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046445
Plasmid#145493PurposeYeast Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046589
Plasmid#145450PurposeYeast Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-GBP-RFP-kanMX6
Plasmid#105160PurposeYeast gene targetingDepositorInsertGBP-RFP-kanMX6
UseTagsGBP-RFPExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry
Plasmid#164917PurposeDivergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizerDepositorInsertP(PGK1)-citrine and P(HIS4)-mCherry
UseTagsExpressionYeastMutationPromoterPGK1 (yeast) and HIS4 (yeast)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorInsertNum1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-NatMx
Plasmid#122897PurposeADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046277
Plasmid#145428PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHES833
Plasmid#90409PurposeExpresses YFP under control of GAL1 promoterDepositorInsertpZ-YFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4782
Plasmid#106141PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
