8,128 results

1. pGBW-m4046687

   Plasmid

   #149148

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAG426GAL-EYFP-ccdB

   Plasmid

   #14347

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG424-ccdB

   Plasmid

   #70124

   Purpose

   Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   None

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EZ-L498

   Plasmid

   #131771

   Purpose

   PPGK1_FUSN_Citrine _PixE_TCYC1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PPGK1_FUSN_Citrine _PixE_TCYC1
   

   Use

   Integration into his3 locus

   Expression

   Yeast

   Promoter

   PGK

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS42K_PCP-VP64_B-PP7

   Plasmid

   #177157

   Purpose

   Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   PCP-VP64
   B-PP7
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   ADH1 promoter and SNR52 promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416 Hsh155 WT

   Plasmid

   #87219

   Purpose

   Plasmid used to maintain viability after deletion of genomic copy of HSH155.

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046268

   Plasmid

   #145423

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJW1670

   Plasmid

   #112044

   Purpose

   CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   OLE1
   

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Mutation

   P319T

   Promoter

   GAL1

   Available Since

   May 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046216

   Plasmid

   #145490

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. MET FLAG103QDProCFPp303

    Plasmid

    #15591

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pPSG465

    Plasmid

    #158587

    Purpose

    Enzymes Bv-CYP76AD5, DODC, ARO4_K229L

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    Bv-CYP76AD5
    DODC
    ARO4_K229L
    

    Expression

    Yeast

    Mutation

    K229L

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CHL1 wt-eGFP

    Plasmid

    #117876

    Purpose

    protein expression

    Depositor

    Geoffrey Chang

    Insert

    CHL1 wt-eGFP (AT5G40090 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ZmMATE2

    Plasmid

    #117094

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    ZmMATE2
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. OsMATE

    Plasmid

    #117093

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtTPT (ORF-HIS)
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AtFRD3

    Plasmid

    #117086

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtFRD3
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT2

    Plasmid

    #98306

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    

    Expression

    Yeast

    Mutation

    ERG20 N127W

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 416Gal-FUS-R514G-YFP

    Plasmid

    #29616

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R514G

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. plIVMD164

    Plasmid

    #160936

    Purpose

    In vivo mRNA display construct for GFP

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
    

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRG236

    Plasmid

    #64534

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge